CHEN Xiao-yun;WANG Hong-xin;L Wen-ping;School of Food Science and Technology,Jiangnan University;

CHEN Xiao-yun%WANG Hong-xin%L Wen-ping%School of Food Science and Technology,Jiangnan University

Finger citron polysaccharides;;Different extraction methods;;Physicochemical properties;;Alcohol dehydrogenase

[Objective] The research aimed to study the effect of different extraction methods on characteristics and alcohol dehydrogenase(ADH) activity of finger citron polysaccharides(FCPs),which provide the references for the development of extraction methods and hepatoprotective activity of FCPs.[Method]FCPs were extracted by five extraction methods,including traditional water extraction(TWE),ultrasonic assisted extraction(UE),microwave assisted extraction(ME),ultrasonic-microwave synergistic extraction(UMSE) and compound enzyme assisted extraction(CEE). The impacts of different extraction methods on the yield of polysaccharides,uronic acid content,molecular weight,monosaccharide composition,IR spectrum and ADH activity were investigated. [Result]The yields of FCPs extracted by TWE and UMSE were(4.26±0.21) % and(4.15±0.14) %,respectively,which were higher than other extraction methods. There were differences in molecular weight of FCPs prepared by different extraction methods. Average molecular weight of polysaccharides prepared by CEE was 617.85 k Da,about 4 times than the polysaccharides by TWE. Five polysaccharides had typical absorption for pyran polysaccharides and α-glycosidic bond. Monosaccharide composition mainly included arabinose,galactose,glucose. The polysaccharides extracted by UMSE had the highest activating effect on ADH,with a percentage activation of(46.58±0.26) %,but polysaccharides extracted by TWE inhibited the activity of ADH.[Conclusion]The polysaccharide prepared by UMSE has good yield,high efficiency and highest activating effect on ADH. The activation of ADH may be due to the molecular weight and galacturonic acid connection.