中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 冷冻家猫附睾精液效果初探 中国畜牧兽医 2009,36 (6) 210-213
Position: Home > Articles > Effect of Cryopreservation on Domestic Cat Epididymal Spermatozoa China Animal Husbandry & Veterinary Medicine 2009,36 (6) 210-213

冷冻家猫附睾精液效果初探

作  者：
徐杨;鲍文玉;郭勇;邬静;倪和民
单  位：
北京农学院动物科学技术系
关键词：
家猫;附睾精液;冷冻
摘  要：
为了筛选出适宜冷冻家猫精液的稀释液、冷冻保护剂和解冻温度,本试验采用家猫附睾精液,分别用2种不同的稀释液,3种不同的冷冻保护剂进行稀释、冷冻和解冻。结果显示,Ⅱ号稀释液冷冻效果优于Ⅰ号稀释液。用乙二醇作为冷冻保护剂,解冻后家猫精子活率达到52.7%±4.9%,畸形率为37.3%±4.2%,顶体完整率为52.4%±4.1%,各项指标均显著高于甘油组和二甲基亚砜组(P<0.05);其中甘油组解冻后活率为35.5%±7.6%,顶体完整率为39.8%±4.4%,高于二甲基亚砜组的33.6%±7.3%和39.1%±4.1%,但两者之间差异不显著(P>0.05)。37℃水浴解冻后顶体完整率为36.4%±8.7%,显著高于30℃水浴解冻后的25.3%±6.7%(P<0.05),但它们之间的活力,畸形率差异不显著,总体上37℃的解冻效果优于30℃。因此,乙二醇和稀释液Ⅱ配合使用冷冻家猫附睾精液效果较好,37℃为较优解冻温度。
译  名：
Effect of Cryopreservation on Domestic Cat Epididymal Spermatozoa
作  者：
XU Yang1,BAO Wen-yu1,GUO Yong1,WU Jing1,2,NI He-min1(1.Department of Animal Science & Technology,Beijing University of Agriculture,Beijing 102206,China;2.College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China)
关键词：
domestic cat;epididymal spermatozoa;cryopreservation
摘  要：
To search the better extender,cryoprotectant and thaw temperature for cryopreservate domestic cat epididymal spermatozoa,the domestic cat epididymal spermatozoa was collected and assessed before and after rapid cryopreservation by two different extenders and three different cryoprotectants respectively.Then,they were thawed in warm water bath either at 37 ℃ or 30 ℃.The results showed that extender II was much more effective than another one.As a cryoprotectant,the glycol was also much better than the glycerol and DMSO(P<0.05).The post-thawing motility and abnormal sperm rate of the frozen/thawed spermatozoa protected by glycol reached 52.7%±4.9% and 37.3%±4.2% respectively.Its intact acrosome rate was 52.4%±4.1%,but there was no significant difference between the group with glycerol and that with DMSO.It indicated that the glycol and extender II was better for cryopreservate domestic cat epididymal spermatozoa,thawing effect at 37 ℃ was much better than that at 30 ℃.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46