Huichao Liu;Wenqing Jia;Du XiaoHua;Han RuiJua

honeysuckle;pollen viability;suitable;staining;ttc staining;pollen germinatio

[Objective] Pollen viability of honeysuckle was determined at different storage conditions,which provided a reference for producing and researching of honeysuckle.[Method] Taken the fresh pollen of honeysuckle as an experimental material,I-KI staining,TTC staining and culture medium germination methods were used to determine its fresh pollen ability.Effects of different storage on the pollen germination were studied.[Result] The results showed that addition different concentration of sucrose and boric acid to culture media had promoted effects on the pollen germination.When the concentrations were 150 and 30 mg/L,the germination rate reached the maximum,which were 75.23% and 52.78%.I-KI staining was not suitable for the determination of pollen viability of honeysuckle,while TTC staining was suitable for it and rates reached 80.46%.[Conclusion] This method is relative correct and simple.It was better mid-low temperature storage(-80 ℃)than cold storage(4 ℃、-23 ℃),it was not suitable for the normal condition.