中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 太子参根际尖孢镰刀菌绝对荧光定量检测方法的建立及其应用 江西农业大学学报 2014 (5) 208-212
Position: Home > Articles > Establishment and Application of qRT-PCR Method for Quantification of Fusarium oxysporum f. sp. in Pseudostellaria heterophylla Rhizosphere Acta Agriculturae Universitatis Jiangxiensis 2014 (5) 208-212

太子参根际尖孢镰刀菌绝对荧光定量检测方法的建立及其应用

作  者：
赵永坡;初雷霞;岳开华;李振方;林文雄
单  位：
福建农林大学
关键词：
太子参;连作障碍;根际微生态;尖孢镰刀菌;qRT-PCR
摘  要：
根据太子参尖孢镰刀菌的测序结果,用设计的一对特异性引物,构建其分子定量检测方法,并进行不同连作年限太子参根际土壤中该尖孢镰刀菌的菌群定量分析,研究结果表明,结果表明:(1)扩增的阳性条带106 bp,特异性、敏感性结果显示,该PCR方法对太子参镰刀菌DNA的最低检测量为0.000 012 ng/μL,而禾谷镰刀菌、茄病镰刀菌、黄曲霉和解淀粉芽孢杆菌的扩增结果均为阴性。(2)运用此方法对该太子参镰刀菌的定量结果表明,尖孢镰刀菌随太子参连作年限的增加而逐年增多。该检测方法具有快速、准确、灵敏度高、易于操作等优点,并且实现了由定性向定量的跨越,可以为太子参根际微生态中该种病原菌的动态变化研究提供方法及依据,也为阐释太子参重茬连作时伴随爆发大规模病害提供了参考。
译  名：
Establishment and Application of qRT-PCR Method for Quantification of Fusarium oxysporum f. sp. in Pseudostellaria heterophylla Rhizosphere
作  者：
ZHAO Yong-po;CHU Lei-xia;YUE Kai-hua;LI Zhen-fang;LIN Wen-xiong;Institute of Agricultural Ecology,Fujian Agricultural and Forestry University;School of Life Sciences,Fujian Agricultural and Forestry University;Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring,Fujian Agricultural and Forestry University;
关键词：
Pseudostellaria heterophylla;;successive cropping obstacle;;rhizosphere microecology;;Fusarium oxysporum;;qRT-PCR
摘  要：
The specific primers of Fusarium oxysporum f. sp. were designed and the molecular quantitative detection method was built to be used to quantify and analyze the Fusarium oxysporum f. sp. in rhizosphere soil under monoculturally cropped Pseudostellaria heterophylla for different years. The results showed that(1) the amplified positive strip was 106 bp;the minimum detectable amount of Fusarium oxysporum f. sp. DNA was0. 000 012 ng /μL with this method and the amplification results of Fusarium graminearum,Fusarium solani,Aspergillus flavus,bacillus amyloliquefaciens were all negative,(2) the quantifying results of Fusarium oxysporum f. sp. with this method suggested that the number of Fusarium oxysporum f. sp. increased with the monoculture years. This detecting method is of accuracy,high sensitivity and easy accessibility,which realizs the leap from qualitative to quantitative. It offers the methods and foundation for analysis of this pathogen in the rhizosphere micro ecology of Pseudostellaria heterophylla,and illuminates the reason for large-scale occurrence of the disease in successively cropped Pseudostellaria heterophylla.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46