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Position: Home > Articles > Determination of Total Flavonoids and Total Saponins in Different Growth Phases and Different Parts of Citrullus lanatus ssp.vulgaris var.megalaspermus Lin et Chao Northern Horticulture 2015 (9) 109-112

籽瓜不同生育期不同部位总黄酮和总皂苷含量测定

作  者:
张彬;田丽萍;张义晖;李欠南;薛琳
单  位:
石河子大学药学院;石河子蔬菜研究所
关键词:
籽瓜;不同生育期;不同部位;总黄酮;总皂苷;分光光度法
摘  要:
以籽瓜为试材,分别以芦丁和葫芦素B为标准品,采用亚硝酸钠-硝酸铝显色法和香草醛-硫酸比色法分别测定籽瓜中总黄酮和总皂苷含量,为籽瓜质量控制提供科学依据。结果表明:总黄酮线性方程为Y=0.0059C-0.0081,R2=0.9994;总皂苷线性方程为Y=0.005C-0.0335,R2=0.9991。数据表明这2种测定方法准确,且重复性好。籽瓜不同部位的总黄酮和总皂苷含量分别在这2种方法下随生长时期变化而变化,根据这些数据可以为籽瓜的综合开发利用提供参考依据。
译  名:
Determination of Total Flavonoids and Total Saponins in Different Growth Phases and Different Parts of Citrullus lanatus ssp.vulgaris var.megalaspermus Lin et Chao
作  者:
ZHANG Bin;TIAN Li-ping;ZHANG Yi-hui;LI Qian-nan;XUE Lin;Pharmacy School,Shihezi University;Shihezi Vegetable Research Institute;
关键词:
Citrullus lanatus;;different growth phases;;different parts;;total flavonoids;;total saponins;;spectrophotometric method
摘  要:
Taking Citrullus lanatus as test material,the contents of total flavonoids and total saponins fromCitrullus lanatus was determined by sodium nitrite-aluminum nitrate chromomeric method and vanillin-sulfuric acid method,which provide a scientific basis for the quality control of Citrullus lanatus.To determinate the contents of total flavonoids and total saponins fromCitrullus lanatus,the rutin and cucurbitacin B were used as standard product.The results showed that,the linear equations of total flavonoids and total saponins determination were respectively Y=0.0059C-0.0081,R2=0.9994 and A=0.005C-0.0335,R2=0.9991.Data showed that the contents of total flavonoids and total saponin in different parts of Citrullus lanatus with growth period changes.The method was accurate and reproducible,which provided a theoretical basis for the comprehensive development and utilization of Citrullus lanatus.

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