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Position: Home > Articles > Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption FOOD SCIENCE 2011,32 (12) 133-138

大孔吸附树脂分离纯化洋葱皮黄酮的研究

作  者:
徐怀德;陈佳;包蓉;刘坤;李晋
单  位:
西北农林科技大学食品科学与工程学院;西北农林科技大学林学院
关键词:
大孔吸附树脂;分离;纯化;洋葱皮;黄酮
摘  要:
以黄酮含量为指标,比较6种大孔吸附树脂对洋葱皮黄酮的吸附和解吸效果。通过静态吸附与解吸实验,筛选出效果较好的X-5树脂进行动态实验研究。结果表明:X-5树脂纯化洋葱皮黄酮的工艺条件为吸附流速2mL/min、上样溶液pH5.0、上样液质量浓度0.5mg/mL、50mL 80%乙醇作为洗脱液,洗脱流速lmL/min。经X-5树脂纯化后,洋葱皮黄酮纯度从7.95%提高到80.78%。三次重结晶后其纯度可达94.5%。该方法简单可行,纯化效果好,适合于工业化生产。
译  名:
Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption
作  者:
XU Huai-de1,CHEN Jia1,,BAO Rong2,LIU Kun1,LI Jin1 (1.College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China; 2.College of Forestry,Northwest A&F University,Yangling 712100,China)
关键词:
macroporous adsorption resin;separation;purification;onion peel;flavonoids
摘  要:
The characteristics of adsorption and desorption of onion peel flavonoids on six kinds of macroporous resins were compared.Static adsorption and desorption experiments indicated that X-5 resin was the best resin for the separation and purification of flavonoids from onion peel.The optimal process parameters for purifying onion peel flavonoids with X-5 resin were sample loading flow rate of 0.5 mg/mL,sample pH 5.0 and 50 mL of 80% ethanol of as eluent at a flow rate of 1.0 mL/min.After the purification,the purity of flavonoids from onion peel was increased from 7.95% to 80.78% and could reach up to 94.5% after further triple recrystallization.The developed method is simple and feasible for industrialization.

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