当前位置: 首页 > 文章 > CD163分子对PRRSV感染猪子宫内膜内皮细胞的影响 河南农业科学 2017 (12) 130-133
Position: Home > Articles > Effects of CD163 on Infection of Porcine Endometrial Endothelial Cells by Porcine Reproductive and Respiratory Syndrome Virus Journal of Henan Agricultural Sciences 2017 (12) 130-133

CD163分子对PRRSV感染猪子宫内膜内皮细胞的影响

作  者:
冯丽丽;燕照玲;陈海燕;段俊枝;杨艳艳;孙怀昌
单  位:
河南省农业科学院动物免疫学重点实验室;扬州大学兽医学院;河南省农业科学院农业经济与信息研究所
关键词:
猪繁殖与呼吸综合征病毒;CD163;病毒受体;猪子宫内膜内皮细胞
摘  要:
为探索猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)感染猪子宫内膜内皮细胞的机制,研究了CD163分子对PRRSV感染猪子宫内膜内皮细胞的影响。将CD163分子转染猪子宫内膜内皮细胞,构建稳定表达CD163分子的猪子宫内膜内皮细胞系,用0.1 MOI的VR2332株PRRSV感染表达CD163分子的猪子宫内膜内皮细胞,收获不同感染时间的子代病毒并测定病毒滴度。结果显示,同一时间点CD163转染细胞组的PRRSV子代病毒滴度与空载体转染细胞组无明显差异。可见,CD163分子对PRRSV感染猪子宫内膜内皮细胞无明显作用。
译  名:
Effects of CD163 on Infection of Porcine Endometrial Endothelial Cells by Porcine Reproductive and Respiratory Syndrome Virus
作  者:
FENG Lili;YAN Zhaoling;CHEN Haiyan;DUAN Junzhi;YANG Yanyan;SUN Huaichang;Institute of Agricultural Economics and Information,Henan Academy of Agriculture Sciences;Key Laboratory of Animal Immunology,Henan Academy of Agriculture Sciences;College of Veterinary Medicine,Yangzhou University;
关键词:
PRRSV;;CD163;;virus receptor;;procine endonetrial endothelial cell
摘  要:
In order to investigate the mechanism of porcine endometrial endothelial cells infected by porcine reproductive and respiratory syndrome virus,the effects of CD163 on infection of porcine endometrial endothelial cells by porcine reproductive and respiratory syndrome virus( PRRSV) were studied. The recombinant CD163 plasmid was transfected into porcine endometrial endothelial cells and transgenic cell line was generated. Transcription of CD163 in transgenic cell line was detected by RT-PCR and immuonfluorescence. Transgenic cell line was infected by 0. 1 MOI PRRSV( VR2332 srain). The virus was harvested at different time points post-infection and titrated by Marc-145 cells. The result showed that there was no significant difference between CD163 transgenic cells and vector transgenic cells. In conclusion,CD163 had no significant effection on infection of porcine endometrial endothelial cells by PRRSV.

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