当前位置: 首页 > 文章 > 电场诱导融合再生两个柑桔种间体细胞杂种(英文) 华中农业大学学报 2000,19 (3) 268-273
Position: Home > Articles > Two Interspecific Somatic Hybrids of Citrus Produced via Protoplast Electrofusion Journal of Huazhong Agricultural University 2000,19 (3) 268-273

电场诱导融合再生两个柑桔种间体细胞杂种(英文)

作  者:
余桂红;邓秀新
单  位:
华中农业大学作物遗传改良国家重点实验室
关键词:
柑桔;电融合;体细胞杂种;RAPD分析;品种改良
摘  要:
通过电场诱导融合方法分别获得了纽荷尔脐橙与粗柠檬、纽荷尔脐橙与夔柑两个组合的体细胞杂种。纽荷尔脐橙 +粗柠檬组合获得了 4 5棵再生植株。对其中的 3 0棵植株进行染色体检查 ,结果表明 ,全为四倍体 ,2n =4x=3 6。过氧化物同工酶及RAPD分析表明再生植株为体细胞杂种。纽荷尔脐橙 +夔柑组合获得了 2 5株再生植株。染色体检查结果表明 ,全为四倍体 ,2n =4x=3 6。RAPD分析表明再生植株为体细胞杂种
译  名:
Two Interspecific Somatic Hybrids of Citrus Produced via Protoplast Electrofusion
作  者:
Yu Guihong\ Deng Xiuxin  (National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070)
关键词:
citrus, protoplast electro fusion, somatic hybrid, RAPD analysis, genetic improvement
摘  要:
Protoplasts of the embryogenic callus of Newhall navel orange ( Citrus sinensis ) were electro fused with mesophyll protoplasts of Rough Lemon ( C. jambhiri ) and Kuigan tangelo ( C. reticulata × C. grandisi ) respectively. 45 and 25 plants regenerated from these 2 combinations respectively. Chromosome counts of 30 plants of the combination of Newhall navel orange +Rough lemon, and 25 of Newhall + Kuigan showed that all of them were tetraploids (2n=4x=36). Peroxidase isozyme and RAPD analysis verified that the tetraploid plants of Newhall + Rough lemon were somatic hybrids. RAPD analysis with primer OPA14 and OPA08 together verified that all of the tetraploids derived from Newhall+ Kuigan were also somatic hybrid plants.

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