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当前位置: 首页 > 文章 > 稀土元素铕对掌叶大黄Ri质粒转化根生长及蒽醌类成分的影响 吉林农业大学学报 2006,28 (5) 70-73
Position: Home > Articles > Effect of Eu~(3+) on Growth and Production of Anthraquinone in Ri Plasmid-Transformed Root and Normal Roots of Rheum palmatum Journal of Jilin Agricultural University 2006,28 (5) 70-73

稀土元素铕对掌叶大黄Ri质粒转化根生长及蒽醌类成分的影响

作  者：
杨世海;陶静;刘晓峰;果德安;郑俊华
单  位：
吉林亚泰(集团)股份有限公司;北京大学药学院;吉林农业大学中药材学院
关键词：
铕;掌叶大黄;Ri质粒转化根;蒽醌类化合物
摘  要：
试验研究了不同质量浓度的稀土元素铕(Eu3+)对掌叶大黄单克隆毛状根DH5c、DH7a和非转化根Nor生物量、蒽醌化合物合成的影响。结果表明:Eu3+浓度对不同类型大黄根的生物量积累作用效果不同。Eu3+对非转化根Nor和转化根DH7a的生物量积累有抑制作用,低浓度的Eu3+对转化根DH5c生物量积累有促进作用。Eu3+浓度对不同类型大黄根的蒽醌产量有不同影响。单克隆毛状根DH7a在Eu3+为0.1 mg/L和0.05 mg/L时蒽醌产量最高。经Eu3+处理的3种类型大黄根中芦荟大黄素和大黄酸高于大黄素、大黄酚和大黄素甲醚。
译  名：
Effect of Eu~(3+) on Growth and Production of Anthraquinone in Ri Plasmid-Transformed Root and Normal Roots of Rheum palmatum
作  者：
YANG Shi-hai~1,TAO Jing~1,LIU Xiao-feng~2,GUO De-an~3,ZHENG Jun-hua~31.College of Chinese Medicinal Material,Jilin Agricultural University,Changchun 130118,China;2.Jilin Yatai Group Co.Ltd.,Changchun 130031,China;3.School of Pharmaceutical Sciences,Peking University,Beijing 100083,China)
关键词：
Eu~(3+);Rheum palmatum;Ri plasmid-transformed root;anthraquinone
摘  要：
The effect of rare-earth elements Eu~(3+) on biomass and anthraquinone production of two hairy clones DH5c,DH7a and normal root Nor cultures of Rheum palmatum was evaluated after various concentration of Eu~(3+) was administrated into culture medium.The results showed that the concentration of Eu~(3+) had different effects on biomass accumulation of roots. Eu~(3+) had inhibitive effect on biomass of Nor and DH7a,and low concentration of Eu~(3+) had promotive effect on biomass of DH5c.Concentration of Eu~(3+) had different effects on anthraquinone production of roots. Combination of DH7a and 0.1 mg/L,0.05 mg/L concentration of Eu~(3+) presented the highest anthraquinone yield.Aloe-emodin and rhein were predominant in five anthraquinones after being treated with rare-earth element Eu~(3+).

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