Position: Home > Articles > Isolation,Identification and Biological Characteristics of a Wild Pleurotus sp.
Shandong Agricultural Sciences
2016,48
(12)
91-94
一株野生侧耳属菌株的分离鉴定与生物学特性
作 者：
兰玉菲;唐丽娜;李秀梅;王庆武
单 位：
泰安市农业科学研究院
关键词：
侧耳属菌株;ITS序列分析;生物学特性;肺形侧耳
摘 要：
采用组织分离的方法从野生侧耳属菌株(Pleurotu ssp.)子实体上分离得到菌株XZG-ts,以其基因组为模板扩增获得该菌株的ITS序列,序列分析结果表明,菌株XZG-ts与Gen Bank中来自于澳大利亚、印度和中国云南的3个肺形侧耳菌株聚为一簇,组内核苷酸一致率为100%,而与侧耳属其他4个种的10个菌株核苷酸一致率为98.10%~99.06%,依此结果将XZG-ts菌株鉴定为P.pulmonarius。进一步研究了不同碳源、氮源、碳氮组合、温度及p H值对菌株XZG-ts菌丝生长的影响,结果表明,最适碳源为果糖,氮源为酵母膏,最佳碳氮源组合为果糖1%、麦芽糖1.0%、酵母膏0.2%、蛋白胨0.3%,最适生长温度为25℃,最适p H值为6。
译 名：
Isolation,Identification and Biological Characteristics of a Wild Pleurotus sp.
作 者：
Lan Yufei;Tang Lina;Li Xiumei;Wang Qingwu;Taian Academy of Agricultural Sciences;
关键词：
Pleurotus sp;;ITS sequence analysis;;Biological characteristic;;Pleurotus pulmonarius
摘 要：
A fungi strain,XZG-ts,collected from the Mountain Tai,was isolated by tissue isolation and the internal transcribed spacers( ITS) was amplified by PCR. In the phylogenetic tree constructed with ITS,XZG-ts formed a branch with 3 isolates belonging to Pleurotus pulmonarius from Australian,India and China with the consistent rate of 100% in intra-group nucleotide. While it was only 98. 10% ~ 99. 06% with other 10 isolates of Pleurotus. Therefore XZG-ts was identified as P. pulmonarius. The effects of carbon source,nitrogen source,carbon-nitrogen combination and p H value on the growth of mycelium were further studied. The results showed the optimum carbon source,nitrogen source,temperature and p H value was fructose,yeast extract,25℃ and 6,respectively. And the optimum carbon-nitrogen combination was 1% fructose,1. 0% maltose,0. 2% yeast extract,0. 3% peptone.
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