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Position: Home > Articles > Cloning and expression profile analysis of odorant receptor gene in Sitotroga cerealella Journal of Huazhong Agricultural University 2018 (1) 46-51

麦蛾气味受体OrCo基因的克隆及其表达模式分析

作  者:
应益益;马敏;陆艳;刘灿;李雪刚;杨凤连
单  位:
华中农业大学植物科技学院/昆虫资源利用与害虫可持续治理湖北省重点实验室;华中农业大学理学院
关键词:
麦蛾;气味受体;基因克隆;序列分析;表达模式
摘  要:
采用RT-PCR和RACE技术,克隆了麦蛾OrCo基因全长序列,并将该基因命名为ScerOrCo。序列分析结果显示,ScerOrCo全长为1 876bp,开放阅读框长1 422bp,编码473个氨基酸,序列中有7个跨膜区。ScerOrCo基因的氨基酸序列与粘虫Mythimna separate嗅觉受体的同源性高达87%。qRT-PCR检测结果表明ScerOrCo只在麦蛾触角中特异性表达,且雌雄虫间表达量无显著差异。
译  名:
Cloning and expression profile analysis of odorant receptor gene in Sitotroga cerealella
作  者:
YING Yiyi;MA Min;LU Yan;LIU Can;LI Xuegang;YANG Fenglian;Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory/College of Plant and Science Technology,Huazhong Agricultural University;College of Science,Huazhong Agricultural University;
关键词:
Sitotroga cerealella;;odorant receptor;;gene cloning;;sequence analysis;;expression patterns
摘  要:
In the present study,the full-length cDNA of the odorant receptor co-receptor gene was cloned from the antennae of Sitotroga cerealella(named ScerOrCo)by using reverse transcription polymerase chain reaction(RT-PCR)and rapid amplification of cDNA ends(RACE).Sequence analysis revealed that the transcript consisted of 1 876 nucleotides and the open reading frame was 1 422 bp,encoding apeptide of 437 amino acids with 7 putative transmembrane domains.The deduced amino acid sequence of ScerOrCo shared the highest similarity with the olfactory receptor of Mythimna separate(87%).The results of quantitative real time RT-PCR showed that the ScerOrCo mRNA was expressed specifically in adult antennae and had no difference between female and male(P>0.05).

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