当前位置: 首页 > 文章 > iTRAQ-based quantitative proteomic analysis reveals key pathways responsible for scurs in sheep (Ovis aries)
农业科学学报 (英文)
2018,17
(8)
Position: Home > Articles > iTRAQ-based quantitative proteomic analysis reveals key pathways responsible for scurs in sheep (Ovis aries)
Journal of Integrative Agriculture
2018,17
(8)
iTRAQ-based quantitative proteomic analysis reveals key pathways responsible for scurs in sheep (Ovis aries)
作 者：
He Xiao-hong;Chen Xiao-fei;Pu Ya-bin;Guan Wei-jun;Song Shen;Zhao Qian-jun;Li Xiang-chen;Jiang Lin;Ma Yue-hui
单 位：
Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China;Enshi Autonomous Prefecture Acad Agr Sci, Inst Anim Sci, Enshi 445000, Peoples R China;Chinese Acad Agr Sci, Inst Anim Sci, CAAS ILRI Joint Lab Livestock & Forage Genet Reso, Beijing 100193, Peoples R China
关键词：
scurs;sheep;iTRAQ;horn development;ECM-receptor interaction pathway
摘 要：
Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2 (RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the present study, we performed an iTRAQ-based quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify the differentially expressed proteins (DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion processes (biological adhesion (P=4.07x 10(-17)) and cell adhesion (P=3.7x 10(-16))), multicellular organismal process (single-multicellular organism process (P=2.06x10(-11)) and multicellular organismal process (P=2.29 x10(-11))) and extracellular processes (extracellular matrix organization (P=4.77x 10(-16)) and extracellular structure organization (P=4.93 x 10(-16))). Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that extracellular matrix (ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from the perspectives of downstream expressed proteins.
相似文章
-
iTRAQ-based quantitative proteomic analysis reveals key pathways responsible for scurs in sheep (Ovis aries) [Xu He, Xiaofei Chen, Yong‐Jin Pu, Weijun Guan, Shen Song, Qianjun Zhao, Xiangchen Li, Lin Jiang, Yuehui M] 农业科学学报(英文版) 2018,17 (8)
