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Position: Home > Articles > Tissue Culture and Fast Generation of Phloxpaniculata Northern Horticulture 2009 (3) 117-118

宿根福禄考组织培养及快繁技术

作  者:
郭旭欣
单  位:
哈尔滨市农业科学院
关键词:
宿根福禄考;组织培养;外植体;愈伤组织
摘  要:
取宿根福禄考带芽茎段为外植体接种在不同培养基上,研究了不同激素组合对外植体的影响,并探索了最适于产生愈伤组织和生根的培养基配方。结果表明:诱导培养基MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+30 g蔗糖;继代增殖培养基MS+6-BA 1.0 mg/L+NAA 0.3mg/L+30 g蔗糖,月增殖倍数可达7.5;生根培养基为:1/2MS+IBA 0.5 mg/L+15 g蔗糖,生根率高且生根快根粗壮;试管苗移入田间,在珍珠岩∶草炭为2∶1的基质中的移栽成活率最高,达88.50%。
译  名:
Tissue Culture and Fast Generation of Phloxpaniculata
作  者:
GUO Xu-xin(Harbin Academy of Agriculture Science,Harbin,Heilongjiang 150070,China)
关键词:
Phlox paniculata;Tissue culture;Explant;Callus tissure
摘  要:
The stem with sprout of Phloxpaniculata was taken to inoculate into different culture medium,and study on the explant infection by various combination of incretion,and explored the most suitable recipe of culture medium to induce callus tissure and produce roots.The result showed that: induce culture medium was MS+6-BA1.0 mg/L+NAA 0.1 mg/L+30 g sugar;continue proliferation culture medium was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L+30 g sugar,the multiple of proliferation can be 7.5;the culture medium of root production was 1/2MS+IBA 0.5 mg/L+15 g sugar,which had high ratio and fast healthy production of root;The most suitable medium was 2perlite∶1grass peat,when the test tube seedlings were transplanted it will get a highest survival ratio,up to 88.50%.

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