中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 杜鹃花表达序列标签资源中的微卫星信息分析 湖北林业科技 2014,43 (2) 11-14
Position: Home > Articles > Analysis of Simple Sequence Repeats Information in Expressed Sequence Tag Resources of Rhododendron simsii Hubei Forestry Science and Technology 2014,43 (2) 11-14

杜鹃花表达序列标签资源中的微卫星信息分析

作  者：
王书珍;张传进;程华;方元平;项俊;郑永良;金卫斌
单  位：
经济林木种质改良与资源综合利用湖北省重点实验室
关键词：
杜鹃花;微卫星;表达序列标签;遗传多样性
摘  要：
从NCBI数据库下载杜鹃花表达序列标签序列1 323条,去冗余后得到912条EST。采用Tandem repeat finder软件分析,最终在61条EST序列中找到65个SSR位点,出现频率为7.127 2%。二碱基重复是杜鹃花EST内SSR的主要重复类型,占66.153 8%;单碱基和三碱基重复次之,分别占据15.384 6%和9.230 8%。四碱基、五碱基、六碱基类型的SSR相对比较少。多达90.769 2%的SSR位点为完美型微卫星重复。这些富含SSR位点的EST序列将为开发微卫星特异性引物,并用于杜鹃花群体生物多样性和遗传结构分析提供理论依据。
译  名：
Analysis of Simple Sequence Repeats Information in Expressed Sequence Tag Resources of Rhododendron simsii
作  者：
Wang Shuzhen;Zhang Chuanjin;Cheng Hua;Fang Yuanping;Xiang Jun;Zheng Yongliang;Jin Weibin;Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization,College of Chemistry and Life Science,Huanggang Normal University;
关键词：
Rhododendron simsii;;microsatellite;;expressed sequence tag;;genetic diversity
摘  要：
A total of 1 323 Rhododendron simsii ESTs downloaded from the NCBI database were analyzed,from which 912non-redundant ESTs were obtained.Sixty-five EST-SSRs distributed in61 ESTs were found by the Tandem repeat finder software with a frequency of 7.127 2%.Especially,dinucleotide repeat was the main type in R.simsii,accounting for 66.153 8%of all SSRs.The mononucleotide and trinucleotide repeats took up to 15.384 6% and 9.230 8%,respectively.By contract,the rest types including tetranucleotide,pentanucleotide and hexanucleotide repeats were the least.Totally,90.769 2%of these SSR loci were perfect repeats.These EST sequences containing SSR loci would facilitate the further development of specific SSR markers,as well as the study of biodiversity and genetic structure of R.simsii.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46