当前位置: 首页 > 文章 > 萝卜-芥蓝异源四倍体F_4和F_(10)世代DNA甲基化变异的MSAP分析 华中农业大学学报 2010,29 (1) 102-106
Position: Home > Articles > Methylation Variation of Different Generations of Artificial Raphanus sativus×Brassica alboglabra Allopolyploids Analysed with MSAP Journal of Huazhong Agricultural University 2010,29 (1) 102-106

萝卜-芥蓝异源四倍体F_4和F_(10)世代DNA甲基化变异的MSAP分析

作  者:
李象松;魏丽华;李炫丽;汪艳杰;王冰;吴江生;龙鸿
单  位:
华中农业大学生命科学技术学院;华中农业大学作物遗传改良国家重点实验室
关键词:
MSAP;异源多倍体;DNA甲基化;5-氮胞苷
摘  要:
以人工合成的萝卜-芥蓝异源四倍体的F4和F10世代为材料,用MSAP法检测2种材料DNA甲基化变异情况,以期为远缘杂交后不同世代间基因组DNA甲基化遗传和变异规律提供实验依据。结果表明:F4代和F10代甲基化程度分别为30.61%和33.10%,共检测12种甲基化变异模式,可以分为5种类型,甲基化变异位点占所有甲基化位点的41.71%。用甲基化抑制剂5-氮胞苷处理F4代,F10代材料,检测到甲基化程度分别为20.67%和25.75%,F代比F代受到了更大冲击,说明不稳定世代对环境的影响更加敏感。
译  名:
Methylation Variation of Different Generations of Artificial Raphanus sativus×Brassica alboglabra Allopolyploids Analysed with MSAP
作  者:
LI Xiang-song1 WEI Li-hua1 Li Xuan-li1 WANG Yan-jie1WANG Bing1 WU Jiang-sheng2 LONG Hong11.College of Life Sciences and Technology,Huazhong Agricultural University,Wuhan 430070,China;2.National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China
关键词:
MSAP;allopolyploids;DNA methylation;5-azacytidine
摘  要:
Methylation variations of artificial F4 and F10 generations of Raphanus sativus×Brassica alboglabra allopolyploid were examined with MSAP.The results showed that the methylation levels of F4,F10 were 30.61% and 33.10%,respectively.Twelve different models of methylation variations were detected,and these models could be divided into 5 types,with 41.71% variable methylation sites.The methylation levels of F4 and F10 after 5-azacytidine treatment were 20.67% and 25.75%,respectively.The results showed that after 5-azacytidine treatment,methylation variations of F10 were stronger than those of F4.

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