作 者：
刘钦来;修世鹏;吕品;唐光峰;张杰;王晓军;赵英健;孙文平
单 位：
泰山医学院病理教研室;泰安市公安局;新泰市人民医院
关键词：
丝光绿蝇;CYTB;地理隔离;遗传多样性;法医学;死亡地点
摘 要：
【目的】对中国多地丝光绿蝇Lucilia sericata种群线粒体细胞色素b(cytochrome b, CYTB)基因进行测序,探究地理隔离对中国丝光绿蝇线粒体细胞色素b基因遗传多样性的影响,并探讨其在法医学上死亡地点推断中的应用。【方法】对2016年采自中国11个地区的丝光绿蝇55头个体的线粒体细胞色素b基因进行PCR扩增和测序;运用MEGA 6.0进行遗传多样性分析、系统发育分析和遗传距离分析,运用Primer 5进行聚类分析,运用WinArl35软件进行遗传分化研究,运用DnaSP 5.0进行单倍型统计分析,运用SPSS进行数据整理。并于2017年采用随机抽样的方法抽取其中2个地区(七台河和楚雄),再捕捉丝光绿蝇进行地域特征单核苷酸多态性(SNP)位点的验证实验。【结果】共获得55条丝光绿蝇线粒体CYTB基因序列(GenBank登录号:MG696659-696663; MG734397-734446),共发现19个变异位点,样本总体核苷酸多态度(Pi)为0.00205,组内平均遗传距离为0.00178。主坐标聚类分析显示所有样本聚为3簇。种群间遗传分化指数Fst有统计学意义的共有6组,范围为0.056～0.350。累计遗传距离和累计纬度差之间具有较高的相关性。序列比对共发现11个地域性私有SNP位点,14个私有单倍型。SNP位点验证实验表明,楚雄样本无新增的SNP位点,七台河样本新增3个SNP位点,其中2个地域性私有SNP位点。【结论】地理隔离对中国丝光绿蝇线粒体细胞色素b基因的遗传多样性具有影响,这种影响可能对法医学上死亡地点的推测有所帮助。
译 名：
Genetic diversity of mitochondrial cytochrome b gene of Lucilia sericata (Diptera: Calliphoridae) populations in China and its implication in forensic medicine for speculating death location
作 者：
LIU Qin-Lai;XIU Shi-Peng;Lü Pin;TANG Guang-Feng;ZHANG Jie;WANG Xiao-Jun;ZHAO Ying-Jian;SUN Wen-Ping;Department of Pathology, Taishan Medical University;Xintai People's Hospital;Tai′an Public Security Bureau Tai′an;
单 位：
LIU Qin-Lai%XIU Shi-Peng%Lü Pin%TANG Guang-Feng%ZHANG Jie%WANG Xiao-Jun%ZHAO Ying-Jian%SUN Wen-Ping%Department of Pathology, Taishan Medical University%Xintai People's Hospital%Tai′an Public Security Bureau Tai′an
关键词：
Lucilia sericata;;CYTB;;geographic isolation;;genetic diversity;;forensic medicine;;death location
摘 要：
【Aim】 To sequence the mitochondrial cytochrome b(CYTB) gene of populations of the green bottle fly, Lucilia sericata from different localities in China, to investigate the effect of geographical isolation on the genetic diversity of L. sericata in China and to assess its application in forensic medicine for speculating death location. 【Methods】 The mitochondrial cytochrome b gene of 55 individuals of L. sericata collected in 2016 from 11 localities in China was amplified by PCR and sequenced. Then we used a variety of biological software for data analysis, including MEGA 6.0 for the analysis of genetic diversity, phylogeny and genetic distance, Primer 5 for cluster analysis, WinArl35 for genetic differentiation analysis, DnaSP 5.0 for haplotype statistical analysis and SPSS for data reduction. In 2017, two of the 11 localities(Qitaihe and Chuxiong) were selected using random sampling, and more L. sericata flies from the two localities were captured to verify the SNP locus of regional features. 【Results】 A total of 55 CYTB sequences(GenBank accession no.: MG696659-696663; MG734397-734446) of L. sericata were obtained with 19 mutation sites. The overall nucleotide polymorphism(Pi) was 0.00205, and the average genetic distance within the group was 0.00178. The main-coordinate clustering analysis showed that all the samples clustered into three clusters. The fixation index(Fst) values in six groups were statistically significant among the populations, ranging from 0.056 to 0.350. There was a high correlation between cumulative genetic distance and cumulative latitude. A total of 11 regional private SNP loci and 14 private haplotypes were found in all the samples. In the SNP verification experiment, there were no new SNP loci in Chuxiong samples, and three new SNP loci in Qitaihe samples, including two regional private SNP loci.【Conclusion】 Geographic isolation has an impact on the genetic diversity of mitochondrial cytochrome b gene of L. sericata in China, and this effect may be helpful to speculate death location in forensic medicine.
