中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 水牛ABCG2基因第9外显子群体遗传特征分析 云南农业大学学报(自然科学) 2012,27 (2) 188-192+197
Position: Home > Articles > Population Genetic Characteristics Analysis of Exon 9 of ABCG2 Gene in Water Buffalo(Bubalus bubalis) Journal of Yunnan Agricultural University(Natural Science) 2012,27 (2) 188-192+197

水牛ABCG2基因第9外显子群体遗传特征分析

作  者：
李卓然;李卫真;苗永旺;李大林;袁跃云;袁峰;刘丽仙;章纯熙
单  位：
云南农业职业技术学院畜牧兽医系;云南农业大学动物科学技术学院;中国农业科学院水牛研究所;云南省家畜改良工作站
关键词：
河流型水牛;沼泽型水牛;三磷酸腺苷结合转运蛋白;第9外显子;PCR-SSCP
摘  要：
三磷酸腺苷结合转运蛋白G超家族成员2(ABCG2)对普通奶牛泌乳性状有直接影响。水牛中该基因的遗传特征与产奶性状间是否有遗传关联目前还不十分清楚。本文采用PCR-SSCP和PCR产物直接测序相结合的技术,对4个河流型水牛群体和9个沼泽型水牛群体共计466个个体的ABCG2基因第9外显子进行了变异检测。结果表明,水牛ABCG2基因第9外显子由251个核苷酸组成,所有的河流型和沼泽型水牛序列相同,未发现SNP位点,但在第9内含子内检测到1个T>C替换(g.SNP44769 T>C)。与GenBank数据库中已发表的牛科物种同源序列比对后发现,数据库中一条河流型水牛序列该外显子第66位核苷酸处有1个G>A替换(c.SNP1018 G>A),导致所编码的氨基酸发生p.V340I的改变;与普通牛、山羊和绵羊该基因第9外显子序列的核苷酸差异位点个数分别为5,8和11个,表明水牛与其他牛科物种间具有较大的遗传差异。本文结果提示ABCG2基因第9外显子在水牛中已纯化固定,且与水牛产奶量性状间无直接关联性。
译  名：
Population Genetic Characteristics Analysis of Exon 9 of ABCG2 Gene in Water Buffalo(Bubalus bubalis)
作  者：
LI Zhuo-ran1,LI Wei-zhen1,MIAO Yong-wang1,2,LI Da-lin3, YUAN Yue-yun3,YUAN Feng1,LIU Li-xian4,ZHANG Chun-xi5(1.College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China; 2.Laboratory for Conservation and Utilization of Bio-resources,Yunnan University,Kunming 650091,China; 3.Domestic Animal Breeding and Crossbreed-improvement Station of Yunnan Province,Kunming 650225,China; 4.Department of Animal Husbandry & Veterinary Medicine,Yunnan Agricultural College of Vocational Education, Kunming 650212,China;5.Buffalo Research Institute,Chinese Academy of Agricultural Science,Nanning 530001,China)
关键词：
river buffalo;swamp buffalo;ATP-binding cassette sub-family G member 2;exon 9;PCR-SSCP
摘  要：
The ATP-binding cassette sub-family G member 2(ABCG2) protein plays a crucial role on the milking traits of cattle.But its population genetic characteristics and association with milk production in buffalo were unclear.In this study,ABCG2 exon 9 of 466 buffalo sampled from 4 river type and 9 swamp type populations were examined using PCR-SSCP and PCR product direct sequencing techniques.The results revealed that sequences of ABCG2 exon 9 in river type and swamp type buffalo were identical,which consist of 251 nucleotides with no SNP discovered,but one T>C substitution(g.SNP44769 T>C) was detected in intron 9.Compared with published data of the bovidae species in GenBank database,one G>A substitution(c.SNP1018 G>A) was found in one river buffalo sequence and lead to the change of the 340th amino acid residues from Valine to Isoleucine(p.V340I) in ABCG2.And the numbers of nucleotide sequence differences among buffalo,cattle,goat and sheep were 5,8,11,respectively,which suggest that there are distinct genetic differences between buffalo and the other bovidae species.The results in the present study indicate that ABCG2 exon 9 has been fixed in buffalo and probably had no direct correlation with the trait of buffalo milk yield.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46