中国农业期刊集成服务平台

当前位置: 首页 > 文章 > Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits 农业科学学报 (英文) 2013,12 (1)

Position: Home > Articles > Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits Journal of Integrative Agriculture 2013,12 (1)

Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits

作者：

Niu Bu-yue;Lan Xiao-ming;Yan Xiao-hong;Di Sheng-wei;Wang Yang;Li Hai-tao;Xiong Yuan-zhu;Wang Xi-biao

单位：

NE Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China;Huazhong Agr Univ, Key Lab Swine Genet & Breeding, Minist Agr, Key Lab Agr Anim Genet Breeding & Reprod,Minist E, Wuhan 430070, Peoples R China

关键词：

pig;MMP-23;molecular cloning;expression;litter size

摘要：

The matrix metalloproteinase 23 (MMP-23), which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 (pMMP-23) gene, including exons 2-8 (GenBank: EU360790), was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 (EU360790: g. 269C>T) and an A/G mutation in exon 4 (EU360790: g. 505A>G), respectively. Association study for these two SNPs with litter size was assessed in three independent populations (Minzhu, Landrace I and Landrace II). Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed (P<0.05) at g. 269C>T locus, and the additive effect was significant (P<0.05); GG sows produced more TNB and NBA than AA pigs in Minzhu (P<0.01) and Landrace breeds (P<0.05) at g. 505A>G locus, and the additive effect was significant (P<0.01 or P<0.05). Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A>G locus might be a useful molecular marker for marker assisted selection (MAS).

相似文章

Effect of Indica Rice Diets Supplemented with Exogenous Enzyme on Growth and Digestion of Pigs Fed Paddy-Based Diets [WANG Min-qi, Xu Zi-rong, SUN Jian-yi] 水稻科学(英文版) 2004,11 (5/6) 336-341
Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size [Liang Jing, Li Na, Zhang Long-chao, Wang Li-gang, Liu Xin, Zhao Ka-bin, Yan Hua, Pu Lei, Zhang Yue-bo, Shi Hui-bi, Zhang Qin, Wang Li-xian] 农业科学学报(英文版) 2016,15 (6)
感染猪放线杆菌(I型)对猪红细胞免疫功能的影响 [左之才, 崔恒敏, 甘梦, 彭西, 邓俊良] 东北农业大学学报 2012,43 (12) 21-25
Genome-wide association study for rib eye muscle area in a Large WhitexMinzhu F-2 pig resource population [Guo Yun-yan, Zhang Long-chao, Wang Li-xian, Liu Wen-zhong] 农业科学学报(英文版) 2015,14 (12)
Genome-Wide Association Study for Certain Carcass Traits and Organ Weights in a Large WhitexMinzhu Intercross Porcine Population [Liu Xin, Wang Li-gang, Liang Jing, Yan Hua, Zhao Ke-bin, Li Na, Zhang Long-chao, Wang Li-xian] 农业科学学报(英文版) 2014,13 (12)
Quantitative trait loci for the number of vertebrae on Sus scrofa chromosomes 1 and 7 independently influence the numbers of thoracic and lumbar vertebrae in pigs [Zhang Long-chao, Liu Xin, Liang Jing, Yan Hua, Zhao Ke-bin, Li Na, Pu Lei, Shi Hui-bi, Zhang Yue-bo, Wang Li-gang, Wang Li-xian] 农业科学学报 (英文) 2015,14 (10)
Molecular characterization and tissue expression profile of the Dnmts gene family in pig [Luo Zong-gang, Zhang Kai, Chen Lei, Yang Yuan-xin, Fu Peng-hui, Wang Ke-tian, Wang Ling, Li Ming-zhou, Li Xue-wei, Zuo Fu-yuan, Wang Jin-yong] 农业科学学报 (英文) 2017,16 (6)
Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs [Chen Liang, Gao Li-xiang, Liu Li, Ding Ze-ming, Zhang Hong-fu] 农业科学学报 (英文) 2015,14 (12)
Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs [Zhao Shuan-ping, Tang Zhong-lin, Zhou Rong, Qu Chang-qing, Zheng Jian-wei, Li Kui] 农业科学学报 (英文) 2014,13 (5)
Fermentation characteristics of Megasphaera elsdenii J6 derived from pig feces on different lactate isomers [Jiang Xiao-lin, Su Yong, Zhu Wei-yun] 农业科学学报 (英文) 2016,15 (7)

导出引用

计量

文章访问数: 5

HTML全文浏览量: 0

PDF下载量: 0

Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits

相似文章

所属期刊

农业科学学报 (英文)

推荐期刊

农业科学学报 (英文)