中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 10株猪流行性腹泻病毒云南分离株的S基因克隆及分子特征 畜牧与兽医 2019 (5) 101-107
Position: Home > Articles > 10株猪流行性腹泻病毒云南分离株的S基因克隆及分子特征 Animal Husbandry & Veterinary Medicine 2019 (5) 101-107

10株猪流行性腹泻病毒云南分离株的S基因克隆及分子特征

作  者：
钱祁昇;朱丽;苏琳琳;张天羽;赵翔玥;郑晓林;吕念词;柴俊;张以芳
单  位：
云南农业大学动物医学院
关键词：
猪流行性腹泻病毒;PEDV;S基因;分子特征;系统发育分析
摘  要：
自2013年来,猪流行性腹泻病(PED)的暴发给云南省养猪业带来严重的经济损失.本研究对2018年来自云南4个地区的42份病料中分离出的6株猪流行性腹泻病毒(PEDV)的部分S基因进行扩增,并结合在2013年所分离到的来自云南4个地区的4株PEDV流行株,进行分子特征和遗传进化分析.结果显示:这2年的分离株和CV777株相比,2018年分离株的核苷酸和氨基酸突变位点增加了12个和7个,且有3个丝氨酸突变都是在COE结构域(69、101和146).系统发育分析表明,2018和2013年分离株的核苷酸和氨基酸的同源性为95.6%~98.8%,95%~98.8%;分别与CV777株相比,核苷酸和氨基酸的同源性差异较小(94.9%~95.5%和92.7%~95.4%;94.5%~95.2%和93.4%~94.2%),亲缘关系都较远.2013年的分离株主要分布在G2b亚群,而2018年的分离株主要分布在G2a和G2c亚群,具有遗传多样性.N-糖基化和磷酸化位点预测分析显示,在216位上预测的N-糖基化位点由NSSI→NSSF,尤其是COE结构域中的丝氨酸取代而导致其可预测磷酸化.这些变异很可能会影响其抗原性和疫苗效果,导致PEDV控制效果不好.本研究阐明了云南部分地区PEDV流行毒株的分子流行病学和遗传特征,为该地区PED的防控奠定理论基础.
作  者：
QIAN Qisheng;ZHU Li;SU Linlin;ZHANG Tianyu;ZHAO Xiangyue;ZHENG Xiaolin;LU Nianci;CHAI Jun;ZHANG Yifang;College of Veterinary Medicine, Yunnan Agricultural University;
单  位：
QIAN Qisheng%ZHU Li%SU Linlin%ZHANG Tianyu%ZHAO Xiangyue%ZHENG Xiaolin%LU Nianci%CHAI Jun%ZHANG Yifang%College of Veterinary Medicine, Yunnan Agricultural University
关键词：
porcine epidemic diarrhea virus;;PEDV;;spike gene;;molecular characterization;;phylogenetic analysis
摘  要：
Since 2013, the outbreak of porcine epidemic diarrhea virus(PEDV) has brought serious economic losses to the pig industry in Yunnan Province. In this study, the some S genes of six strains of PEDV isolated from 42 samples from four regions in Yunnan in 2018 were amplified. Combined with four PEDV strains isolated from four regions of Yunnan in 2013, all these strains were analyzed for molecular characteristics and genetic evolution. The results showed that, compared with the CV777 strain, the nucleotides and amino acid mutations of the 2018 isolates and the 2013 isolates were increased by 12 sites and 7 sites, respectively; and they had three serine mutations in the COE domain(69, 101 and 146). Phylogenetic analysis showed that the homology between nucleotide and amino acid of the 2018 and 2013 isolates was 95.6% and 98.8%, respectively. Separately compared with the CV777 strain, the homologies of nucleotide and amino acid with it were 94.9%～95.5% and 92.7%～95.4%, and 94.5%～95.2% and 93.4%～94.2%, respectively, with smaller differences in homology; which indicated a far-away relationship between the isolates. The 2013 isolates were mainly distributed in the G2b subgroup, while the 2018 isolates were mainly distributed in the G2a and G2c subpopulations, showing a genetic diversity. The prediction of N-glycosylation and phosphorylation sites showed various changes, especially with the predicted N-glycosylation site at position 216 being replaced by NSSI→NSSF, and serine substitution in the COE domain resulting in predictable phosphorylation. These mutations were likely to affect the antigenicity and vaccine efficacy of the strains, resulting in poor PEDV control. The present study revealed the molecular epidemiology and genetic characteristics of the PEDV strains in some areas of Yunnan, and provided further insight into PEDV outbreaks in these areas, which was helpful for formulation of measures for preventive and control of PEDV.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46