Position: Home > Articles > Detection on the Halothane Gene in Fengqiao Breeding Pigs of Jiaxing by PCR-RFLP Technique
Journal of Anhui Agricultural Sciences
2009,37
(24)
11437-11438
PCR-RFLP技术检测嘉兴凤桥种猪氟烷基因
作 者：
杜晓婷;刘锐;张水明;杨卫景;吴正红;陆建中;金建芳
单 位：
嘉兴南湖区畜牧兽医局;浙江工业大学;嘉兴职业技术学院;嘉兴正宏生物科技有限公司;嘉兴凤桥畜牧兽医站;嘉兴凤桥农业技术服务中心
关键词：
凤桥;猪;氟烷基因;PCR-RFLP
摘 要：
[目的]为凤桥镇通过合理利用氟烷基因提高猪肉品质奠定理论基础。[方法]从嘉兴县凤桥镇的规模化养猪场随机抽取种猪血样,通过PCR-RFLP技术进行氟烷基因检测。[结果]HhaⅠ内切酶的识别位点为5′GCG↓C3′。PCR扩增产物是含RYR1基因第1 843位点长度为659 bp的片段。如果猪的基因型为NN,由于未发生胞嘧啶C到胸腺嘧啶T的突变,可以看到2条带。如果猪的基因型为nn,由于发生了胞嘧啶C到胸腺嘧啶T的突变,只能看到1条带。如果猪的基因型为Nn,可以看到3条带。在长白猪、杜洛克猪和大约克猪3个供试品种中没有检测出氟烷基因隐性纯合子(nn),其n等位基因频率分别为2.5%、1.9%和1.6%。[结论]建议在凤桥镇利用的氟烷基因杂合子(Nn)的公系和氟烷基因显性纯合子(NN)的母系开发猪的胴体优势。
译 名：
Detection on the Halothane Gene in Fengqiao Breeding Pigs of Jiaxing by PCR-RFLP Technique
作 者：
DU Xiao-ting et al (Zhejiang University of Technology,Hangzhou,Zhejiang 310019)
关键词：
Fengqiao;Pig;Halothane gene;PCR-RFLP
摘 要：
[Objective] The purpose was to lay a theoretical foundation for enhancing pork quality through utilizing halothane gene reasonably in Fengqiao town.[Method] The blood samples were extracted from breeding pigs randomly in the large-scale piggeries of Fengqiao town in Jiaxing County and the halothane gene was detected by PCR-RFLP technique.[Result] The recognition site of endonuclease Hha Ⅰ was 5′GCG↓C3′.The PCR amplified product was a segment containing the 1843rd site of gene RYR1 and its length was 659 bp.If the genotype of pig was NN,there would be 2 bands appeared for that there was no mutagenesis from cytosine C to thymine T occurred.If the genotype of pig was nn,there would be only 1 band appeared for that there was mutagenesis from cytosine C to thymine T occurred.If the genotype of pig was Nn,there would be 3 bands appeared.There was no allozygote(nn) of halothane gene detected out in the 3 tested varieties of landrace pig,duroc pig and yorkshire pig and their frequences of allele gene n were 2.5%,1.9% and 1.6% resp.[Conclusion] It was suggested that the carcass advantage of pig should be developed by using male lines with heterozygote(Nn) of halothane gene and female lines with dominant homozygote(NN) of halothane gene in Fengqiao town.
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