单 位：
石河子大学食品学院;新疆农业科学院微生物应用研究所
关键词：
水生拉恩菌;低温β-半乳糖苷酶;诱变选育;亚硝基胍
摘 要：
以野生低温β-半乳糖苷酶产生菌水生拉恩菌(Rahnella aquatilis)14-1为出发菌株,通过亚硝基胍(NTG)诱变及低温驯化,采用选择性平板初筛和摇瓶复筛,筛选出一株产酶活力比原始菌株提高54%的突变株,该突变株经传5代培养,产酶特性稳定。
译 名：
Screening of High-producing Cold-adapted β-D-Galactosidase Strain by NTG Mutagenesis
作 者：
Liu Wenyu1,2 Shi Yingwu2 Wang Xingqin2,3 Lou Kai2(1Collge of Food,Shihezi University,Shihezi 832003;2Institute of Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091;3Collge of Food Science,Xinjiang Agricultural University,Urumqi 830052)
关键词：
Rahnella aquatilis Cold-adapted β-D-Galactosidase Mutagenesis Nitrosoguanidine
摘 要：
A wide strain Rahnella aquatilis 14-1 which can be able to produce cold-adapted β-D-Galactosidase,is mutated with nitrosoguanidine at low temperature,by using selectivity plate initial screening and fermentation experiments in shake flask.One strain was obtained with high-yield of cold-adapted β-D-Galactosidase,adn the enzyme productivity of which has increased by 54%.Characteristics of enzymes production in mutated strain was kept stable by 5 times subculture.
