中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 检测牛血吸虫病的重组LHD-Sj23蛋白乳胶凝集试验的建立湖北农业科学 2009,48 (9) 2061-2065

Position: Home > Articles > Establishment of A Recombinant LHD-Sj23 Protein Based Latex Agglutination Test for Diagnosis of Bovine Schistosomiasis Hubei Agricultural Sciences 2009,48 (9) 2061-2065

检测牛血吸虫病的重组LHD-Sj23蛋白乳胶凝集试验的建立

作者：

赵清兰;李友;聂浩;赵俊龙

单位：

华中农业大学动物医学院

关键词：

牛血吸虫病;重组LHD-Sj23蛋白;乳胶凝集试验;诊断

摘要：

利用原核表达的日本血吸虫重组LHD-Sj23蛋白作为抗原致敏乳胶,建立诊断牛血吸虫病的乳胶凝集试验(LAT)。用制备的乳胶抗原分别检测牛结核杆菌、牛泰勒虫、牛巴贝斯虫、牛口蹄疫、牛边虫、牛传染性支气管炎阳性血清,结果均为阴性,说明建立的乳胶凝集方法具有良好的特异性。用所建立的LAT方法与ELISA方法同时检测169份牛血清,结果表明,建立的LAT方法的特异性为94.29%,敏感性为93.10%,两种方法的符合率为94.08%(P>0.05),差异不显著。用同一批乳胶抗原检测7份牛血清,5次检测结果一致,说明致敏的乳胶抗原是稳定的。保存期试验结果表明,致敏乳胶在25℃可保存1个月,在4℃可保存3个月。这表明建立的LAT方法稳定、特异性强、灵敏度高、操作简便快速,无需昂贵仪器,适宜在临床上推广应用。

译名：

Establishment of A Recombinant LHD-Sj23 Protein Based Latex Agglutination Test for Diagnosis of Bovine Schistosomiasis

作者：

ZHAO Qing-lan a,LI Youa,b,NIE Haoa,b,ZHAO Jun-long a,b(Huazhong Agricultural University a.College of Animal Science and Veterinary Medicine;b.National Key Laboratory of Agricultural Microbiology,Wuhan 430070,China)

关键词：

bovine schistosomiasis;recombinant LHD-Sj23 protein;latex agglutination test(LAT);diagnosis

摘要：

The purified recombinant LHD-Sj23 protein coupled with latex microspheres was used to develope a latex agglutination test(LAT) for diagnosis of bovine schistosomiasis.Specificity test showed that all the serum samples of bovine tuber-culosis,bovine theileriosis,bovine babesiosis,bovine foot-and-mouth disease,bovine anaplasmosis and bovine infectious bronchitis were negative to this LAT.169 serum samples were detected by LAT and ELISA at the same time.The results showed that the specificity for the LAT was 94.29%,the sensitivity was 93.10%,the coincidence rate of both methods was 94.08%,no significant difference was found between them(P>0.05).Seven serum samples were detected with the same lot latex antigen for five times,no difference was found.The latex antigen could be preserved at 25℃ for one month and at 4℃ for three months.The results indicated that this LAT method was specific,sensitive,simple,rapid,and suitable for clinical diagnosis of bovine schistosomiasis.

相似文章

1例牛血吸虫病的防治 [付会庭] 当代畜牧 2018 (14) 23-24
间接血凝试验(IHA)应用于牛血吸虫病诊断效果的观察 [刘雪芹] 中国动物保健 2020,22 (12) 79-80
牛血吸虫病防治效果分析 [刘芬] 中国动物保健 2018 (5) 47-48
牛血吸虫病的临床防治经验探讨 [孙红艳] 当代畜牧 2017 (11) 63-64
用日本血吸虫重组抗原诊断牛血吸虫病的研究 [周伟芳, 林矫矫, 朱传刚, 石耀军, 刘金明] 中国兽医科学 2008,38 (6) 489-493
牛血吸虫病防治 [王龙] 草业与畜牧 2012 (9) 52-53
“九0安”对吡喹酮治疗牛血吸虫病副反应的抑制效果观察 [杨合仓] 江西农业科技 1992 (6) 34-35
牛血吸虫病的流行与诊治 [沈强] 兽医导刊 2016 (6) 100
吡喹酮治疗牛血吸虫病效果观察 [马亭] 中国畜禽种业 2021,17 (10) 130-131
粪孵法与斑点酶标法检测牛血吸虫病浅析 [朱贞友] 四川畜牧兽医 2000,27 (9) 32

导出引用

计量

文章访问数: 7

HTML全文浏览量: 0

PDF下载量: 0

检测牛血吸虫病的重组LHD-Sj23蛋白乳胶凝集试验的建立

相似文章

所属期刊

湖北农业科学

推荐期刊

中国动物保健

当代畜牧