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Position: Home > Articles > The in vitro growth effect of three kinds chinese traditional medicines on helpful microbe of intestines Journal of China Agricultural University 2003,8 (5) 35-38

常见中草药对两种肠道有益菌体外生长的影响

作  者:
李平兰;时向东;吕燕妮;江志杰;沈清武;马长伟
单  位:
河南农业大学农学院;中国农业大学食品科学与营养工程学院
关键词:
中草药;两歧双歧杆菌;嗜酸乳杆菌;体外生长
摘  要:
为了解中草药对机体肠道有益菌两歧双歧杆菌、嗜酸乳杆菌体外生长的影响 ,探讨中草药的使用前景。选取清热解毒类的板蓝根、柴胡、连翘 ,泻下类的厚朴、大黄、芒硝 ,补益类的枸杞子、地黄、五味子共 9种常见中草药制成浸提液 ,添加于有益菌体的外生长培养基中 ,采用亨盖特厌氧滚管技术活菌计数。结果显示清热解毒类中药在 3种试验浓度下对两歧双歧杆菌的生长没有显著影响 ,对嗜酸乳杆菌均有抑制作用 (P <0 .0 5 ) ,但不同浓度下抑制作用不同 ;泻下类中药均强烈抑制 2类有益菌的生长 (P <0 .0 1或P <0 .0 5 ) ,且随着浓度的增加抑制作用增强 ;而补益类中药对 2种试验菌均有明显的促进生长作用 (其中对双歧杆菌P <0 .0 5 ) ,且随着浓度增加 ,促进效果增强。因此认为 :在调节肠道微生态平衡方面发挥一定的作用。
译  名:
The in vitro growth effect of three kinds chinese traditional medicines on helpful microbe of intestines
作  者:
Li Pinglan1, Shi Xiangdong2, Lü Yanni1, Jiang Zhijie1, Shen Qingwu1, Ma Changwei1 (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; (2. College of Agriculture Botany Science, Henan Agricultural University, Zhengzhou 450002, China)
关键词:
Chinese traditional medicine(CTM); Bifidobacterium bifidum; Lactobacillus acidophilus; the in vitro growth
摘  要:
To investigate the in vitro growth effect of 9 common CTMs on Bifidobacterium bifidum and lactobacillus acidophilus. These CTMs include Radix isatidis, Radix bupleuri, Radix rhei, Mirabilitum depuratum, Cortex magnoliae, Fructus forsythiae, Fructus lycii, Fructus schizandrao, Rhizoma rehmanniae. Using the Hungate-anaerobic technology to enumerate these two strains of bacteria which grew in media composed of CTMs extraction. The effect of the first three CTMs to Bifidobacterium bifidum is not obvious, but the effect on L. acidophilus is inbitory (P< 0.05), and the inhibition is not in accordance with different concentrations. The other three of the forth to sixth CTMs strongly inhibited the growth of two bacteria in according to the concentration (P<0.01 or P<0.05). The last three CTMs obviously improved the growth in line with concentration(especially to Bifidobacterium bifidum P<0.05). CTMs not only can play the role of curing and guarding against disease, but also can improve the immunity of body. To some extent, CTMs may replace the using of antibiotic to play an important role on intestinal microecology balance.

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