当前位置: 首页 > 文章 > 培养时间对柱状嗜纤维菌脂多糖蛋白质成分和免疫原性的影响 华中农业大学学报 2000,19 (2) 159-162
Position: Home > Articles > Influence of Culture Time on the Protein Composition and Antigenicity of Lipopolysaccharide from Cytophaga columnaris Journal of Huazhong Agricultural University 2000,19 (2) 159-162

培养时间对柱状嗜纤维菌脂多糖蛋白质成分和免疫原性的影响

作  者:
陈昌福;曾妍雄;楠田理一
单  位:
日本国福山大学工学部海洋生物工学科;华中农业大学水产学院
关键词:
柱状嗜纤维菌;脂多糖;翘嘴鳜;免疫原性
摘  要:
从培养不同时间后的柱状嗜纤维菌中提取菌体脂多糖 (LPS) ,测定了其中蛋白质含量及成分的变化 ,并分别作为免疫原接种翘嘴鳜对其免疫原性进行了比较。结果表明 ,培养时间对菌体LPS中蛋白质含量没有明显影响 ,对其蛋白质成分则有一定影响 ,而菌体LPS中蛋白质成分与其免疫原性有关 ,从培养 36h的菌体中提取的菌体LPS的免疫原性最强
译  名:
Influence of Culture Time on the Protein Composition and Antigenicity of Lipopolysaccharide from Cytophaga columnaris
作  者:
Chen Changfu 1) Zeng Yanxiong 1) Riichi Kusuda 2) ( 1) Huazhong Agricultural University, Wuhan 430070, China; 2) Fukuyama University, Hiroshima, 729?02, Japan)
关键词:
Cytophaga columnaris , lipopolysaccharide, Siniperca chautsi , antigenicity
摘  要:
The lipopolysaccharide (LPS) was extracted from Cytophaga columnaris strain SC?5 and SC?6 which had been cultured for different time. Then the content and composition of the protein in LPS was determined using Lowry's method and polyacrylamide gel disc electropharesis method, respectively. The antigenicity of LPS were examined by vaccinating mandarinfish ( Siniperca chautsi Basilewsky). The experimental result showed that the cultune time had no significant effects on the content of protein in LPS, but the composition of LPS were significantly different. The LPS extracted from Cytophaga columnaris which had been cultured for 36 hours has the highest antigenicity.\;

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