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当前位置: 首页 > 文章 > 芹菜衔接蛋白基因AgMu2的克隆与表达分析 园艺学报 2014,41 (7) 1369-1378
Position: Home > Articles > Cloning and Expression Profile Analysis of the AgMu2 Gene of Adaptor Complex from Celery Acta Horticulturae Sinica 2014,41 (7) 1369-1378

芹菜衔接蛋白基因AgMu2的克隆与表达分析

作  者：
王广龙;王枫;徐志胜;蒋倩;谭国飞;熊爱生
单  位：
作物遗传与种质创新国家重点实验室;农业部华东地区园艺作物生物学与种质创制重点实验室;南京农业大学园艺学院
关键词：
芹菜;AP-2复合体;AgMu2基因;克隆;实时定量PCR;基因表达
摘  要：
为研究芹菜(Apium graveolens)衔接蛋白(Adaptor protein)复合体AP-2中的μ2亚基的功能,以‘六合黄心芹’和‘美国西芹’为试验材料,采用RT-PCR方法获得AgMu2基因。序列分析表明:AP-2复合体AgMu2基因全长1 317个核苷酸,编码438个氨基酸。推测其蛋白质分子量为49.30 kD,pI为9.28。进化分析表明,来自‘美国西芹’的AgMu2亚基在植物间进化具有高度保守性,与葡萄Mu2进化关系最为接近。空间结构分析表明,从芹菜中分离的AgMu2亚基由5个螺旋和26个延伸主链构成,两个平行的β延伸主链构成的平面区域可能含有识别YXXΦ结构的位点。实时定量荧光PCR显示,芹菜中AgMu2基因主要在叶中表达,具有明显的组织特异性,同时该基因可响应低温、高温、干旱和盐胁迫等多种逆境信号,2个品种间该基因响应的时间和强度也具有显著的差异,显示了该基因功能的多样性。
译  名：
Cloning and Expression Profile Analysis of the AgMu2 Gene of Adaptor Complex from Celery
作  者：
WANG Guang-long;WANG Feng;XU Zhi-sheng;JIANG Qian;TAN Guo-fei;XIONG Ai-sheng;State Key Laboratory of Crop Genetics and Germplasm Enhancement,Ministry of Agriculture Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,College of Horticulture,Nanjing Agricultural University;
关键词：
celery;;AP-2 complex;;AgMu2 gene;;clone;;quantitative real-time PCR;;gene expression
摘  要：
To investigate the functions of the μ2 subunit of adaptor protein complex from celery(Apium graveolens),the gene AgMu2,encoding the μ subunit from AP-2 complex,was isolated from two celery cultivars‘Liuhe Huangxinqin'and‘Meiguo Xiqin'by RT-PCR method. Sequence analysis indicated that the length of AgMu2 gene was 1 317 bp,which encoded 438 amino acids. It is predicted that the molecular mass of its protein was 49.30 kD,and pI was 9.28. Amino acid sequence comparison showed that the AgMu2 from‘Meiguo Xiqin'existed high evolutionary conservation among different species,and its evolutionary relationship was close to grape(Vitis vinifera). Analysis on three-dimension structure implied that the subunit was composed with 5 helixes and 26 extended strands. The tyrosine based signal YXXΦ binded to a site on the surface of two parallel β-sheet strands. Quantitative real-time PCR analysis demonstrated that the AgMu2 gene was tissue-specific and mainly expressed in leaf. Besides,this gene may be involved into response to low temperature,high temperature,drought,salt stress and a variety of other adverse signals. Significant differences also existed in response time and intensity between the two cultivars,revealing the multiple functions of the subunit gene in celery.

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