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当前位置: 首页 > 文章 > 高效液相色谱仪检测鸡组织器官喹烯酮残留方法的建立 动物医学进展 2004,25 (5) 100-101
Position: Home > Articles > HPLC Determination of Quinocetone in Chicken Tissues Progress in Veterinary Medicine 2004,25 (5) 100-101

高效液相色谱仪检测鸡组织器官喹烯酮残留方法的建立

作  者：
李剑勇;李金善;徐忠赞;赵荣材;苗小楼;张继瑜;鲁润华
单  位：
中国科学院兰州化学物理研究所;中国农业科学院兰州畜牧与兽药研究所农业部新兽药工程重点实验室
关键词：
喹烯酮;鸡;组织器官;HPLC
摘  要：
建立用高效液相色谱法测定鸡组织中喹烯酮浓度的方法,用于监测鸡组织器官中喹烯酮的残留。方法:采用Prodigy 5μPhenyl-3色谱分析柱,Waters TM486可变波长紫外检测器,甲醇-水(70:30)为流动相。组织样品用乙酸乙酯、乙腈、正已烷、氯仿等试剂提取处理纯化后进样,在312 nm波长处检测,流速为1.0 mL／min。标准曲线的线性范围:脂肪、肾脏和肌肉均为0.005-0.080μ／g;肝脏为0.005-0.500μ／g。方法平均回收率:肌肉为(99.23±2.75)％;脂肪为(105.15±8.65)％;肝脏为(93.55±11.16)％;肾脏为(104.10±9.80)％。精密度考察喹烯酮的日内各组织各浓度的RSD(相对标准差)均小于5.1％,日间测得各组织各浓度的RSD均小于8.5％。本方法灵敏度高,重复性好。
译  名：
HPLC Determination of Quinocetone in Chicken Tissues
作  者：
LI Jian-yong, LI Jing-shan, XU Zhong-zan, ZHAO Rong-cai ,MIAO Xiao-lou, ZHANG Ji-yu, LU Run-hua(Lanzhou Institute of Animal Husbandry and Veterinary Pharmaceutics Sciences,Chinese Academy of Agricultural Sciences, Lanzhou,Gansu(730050,China; Lanzhou Institute of Physics and Chemistry,Chinese Academy of Sciences, Lanzhou,Gansu,730000,China;Chengdu Institute of Biology, Chinese Academy of Sciences ,Chendu, Sichuan,610046, China)
关键词：
quinocetone; chicken; tissues; HPLC
摘  要：
A HPLC method is described for the analysis of quinocetone in chicken tissues. Prodigy 5 μ Phenyl-3 column (4. 6 mm±250 mm 5 μ micron) was used. The mobile phase consisted of methanol-water (70 : 30). A flow rate of mobile phase is 1. 0 mL/min. A waters model 480 instrument was used throughout the experiment. The absorption wavelength of determination is 312 nm. Cure regression equation: fat,Y=4±10-5X-0. 0293, R2=0. 9944(n=5). The ranges of quinocetone calibration curves were 0. 005-0. 080 μg/g;liver,Y=3±10-5X - 0. 02,R2 = 0. 9926(n=8). The ranges of quinocetone calibration curves were 0. 005-0. 500 μg/g;kidney,Y= 4±10-4X-0. 0394,R2 = 0. 9933(n=10). The ranges of quinocetone calibration curves were 0. 005-0. 800μg/ g;muscle,Y=6±10-5X-0. 0631,R2 = 0. 9926(n=6). The ranges of quinocetone calibration curves were 0. 005 -0. 080 μg/g. The lowest detectable concentration of quinocetone was 0. 005 μg/g for fat , liver, kidney and muscle. While the average recovery of the method was (99. 23 ±2. 7)% in muscle; (105. 15 ±8. 65)% in fat; (93. 55 ± 11.16)% in liver;l04. 10±9. 80% kidney. The RSD of intra-day of all concentrations and all tissues was all less than 5. 1%,those of inter-day of all concentrations and all tissues was all less than 8.5%, The method is higher sensitivity and good repeat.

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