中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 杨山牡丹和牡丹种间杂交后代的DNA分子证据 林业科学研究 2004,17 (6) 17-22
Position: Home > Articles > DNA Molecular Evidences of the Inter-specific Hybrids between Paeonia ostii and P.suffruticosa Based on ISSR Markers Forest Research 2004,17 (6) 17-22

杨山牡丹和牡丹种间杂交后代的DNA分子证据

作  者：
索志立;周世良;张会金;张治明
单  位：
中国科学院植物研究所系统与进化植物学开放实验室;中国科学院植物研究所
关键词：
杨山牡丹;牡丹;杂交;ISSR标记;DNA指纹图谱;品种鉴定
摘  要：
以杨山牡丹作母本,分别以牡丹品种'赵粉'(PaeoniasuffruticosaAndr.cv.'ZhaoFen')和'紫二乔'(P suffruticosacv.'ZiErQiao')作父本,进行人工杂交,获得了杂交后代。利用DNAISSR标记技术构建的亲子代DNA指纹图谱显示,在杂交后代中检测到了分别来自双亲的特征带。建立起来的专用于牡丹研究的ISSR标记技术方法可以用于牡丹杂交种的苗期快速鉴定。
译  名：
DNA Molecular Evidences of the Inter-specific Hybrids between Paeonia ostii and P.suffruticosa Based on ISSR Markers
作  者：
SUO Zhi-li~1,ZHOU Shi-liang~2,ZHANG Hui-jin~1,ZHANG Zhi-ming~1 (1.Institute of Botany,Chinese Academy of Sciences,Beijing100093,China; 2.Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing100093,China)
关键词：
Paeonia ostii;P.suffruticosa;crossing;ISSR Marker;DNA fingerprinting;cultivar identification
摘  要：
Paeonia ostii was believed to be one of the main progenitors of the Chinese tree peony cultivars,and hybridization was the most important pathway for incorporation of genetic composition of P.ostii.However,no experimental evidence had been reported till now.In this study,P.ostii was used as a maternal parent crossing with other two Chinese tree peony cultivars which were used as paternal parents.The relationships between the F_1 hybrids and their parents were analyzed by means of DNA fingerprinting based on ISSR (Inter-simple sequence repeats) markers,and ISSR fragments typically inherited from both parents were detected in the genomes of the F_1 generation.This study also indicated that ISSR marker was suitable for identification of F_1 hybrids in P.suffruticosa at seedling stage.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46