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Position: Home > Articles > Identification and Expression Profile of Acetyl CoA Carboxylase(ACC) in Cocos nucifera Journal of Anhui Agricultural Sciences 2017,45 (35) 128-129

椰子乙酰CoA羧化酶(ACC)基因的鉴定及表达分析

作  者:
肖勇;雷新涛;王永;曹红星;石鹏;金龙飞;夏薇
单  位:
中国热带农业科学院椰子研究所
关键词:
椰子;转录组;ACC基因;实时定量PCR
摘  要:
[目的]克隆椰子的乙酰Co A羧化酶(ACC)基因.[方法]通过已发表的椰子转录组注释结果,鉴定潜在的椰子ACC基因.分析ACC基因在椰子果肉和嫩叶混合样转录组中的RPKM值,同时,采用实时定量PCR技术研究5个高RPKM值的ACC基因在椰子果肉发育的不同时期的表达情况.[结果]共获得21个椰子的ACC基因,这21个ACC基因在椰子果肉和嫩叶混合样品中有较高的表达量,4个ACC基因(Unigene7806、Unigene47155、Unigene7847和CL185.Contig1)在椰子果肉发育9个月时表达量最高.[结论]该研究为解析椰子脂肪酸积累的分子机制提供了前期工作基础.
译  名:
Identification and Expression Profile of Acetyl CoA Carboxylase(ACC) in Cocos nucifera
作  者:
XIAO Yong;LEI Xin-tao;WANG Yong;Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences;
关键词:
Cocos nucifera;;Transcriptome;;Acetyl CoA carboxylase;;Real-time quantitative PCR
摘  要:
[Objective]To clone acetyl Co A carboxylase( ACC) genes in Cocos nucifera. [Method] Based on the coconut transcriptome data,to identify potential coconut ACC genes. We examined the RPKM value of ACC genes in coconut leaf and fruit transcriptome. Real-time quantitative RT-PCR was applied to test expression level of 5 ACC genes in different developmental stages of coconut fruit, which had highest RPKM value in coconut fruit. [Result]A total of 21 coconut ACC genes were identified. The 21 ACC genes showed high expression level in a mixture sample from coconut pulp. 4 ACC genes( Unigene7806,Unigene47155,Unigene7847 and CL185. Contig1) showed the highest expression during the development of the coconut pulp for 9 months. [Conclusion]These study provided basic work for elucidating the molecular mechanism of fatty acid biosynthesis of coconut.

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