Study on Drought Tolerance of Wheat Seedlings Induced by Plant Root-promoting Bacteria

ZHAO Huiyun;QI Mingyang;DANG Changxi;LIN Huan;SHI Jiabing;YE Yuxiu;WANG Feibing;CHEN Xinhong;College of Life Science and Food Engineering,Huaian Institute of Technology;

plant root-promoting bacteria;;wheat seedling stage;;drought stress;;drought tolerance

[Purpose]To study the drought tolerance of wheat seedlings treated with plant root-promoting bacteria(PGPR).[Method]Commercial wheat variety‘Huaimai 33’seeds were sown and grew in pots.At the tillering stage,the plants were treated with root-promoting bacteria:Bacillus subtilis AR,B.subtilis SM,B.subtilis XY,and combination of the three at AR∶SM∶XY=10∶10∶1Control groups were treated with water(CK_1)and LB solution(CK_2).The plants were then introduced to drought stress condition for 15 days,and plant morphological and physiological characteristics were tested to evaluate drought-tolerance.[Result]Under drought stress,the root-shoot ratio leaf relative water content,chlorophyll content,proline content and root activity of wheat seedlings treated with different root-promoting bacteria were significantly higher than CK,while relative conductivity and malondialdehyde(MDA)content was significantly lower than CK.Our experiments indicated that the enhanced drought tolerance could be achieved by inoculating root-promoting bacteria The inducement varies among the bacteria tested.[Conclusion]Wheat seedlings treated with the combination of three PGPR’s have the strongest drought tolerance.