Study on detection method of arbutin in pomegranate juice concentrates by ultra performance liquid chromatography-tandem mass spectrometry

HE Qiang,KONG Xiang-hong,LI Jian-hua,YUE Ai-shan,WU Shuang-min(Technical Center,Shaanxi Entry-exit Inspection and Quarantine Bureau,Xi'an 710068,China)

pomegranate juice concentrate;arbutin;solid phase extraction;ultra performance liquid chromatography-tandem mass spectrometry

A method was developed for the determination of arbutin in pomegranate juice concentrate by solid phase extraction(SPE)and ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Samples were diluted by water and then cleanup with HLB and aminopropyl SPE columns.UPLC was performed on a BEH Amide column(100mm×2.1mm,1.7μm)using a gradient acetonitrile-water system as mobile phase,and MS/MS was performed with multiple reation monitoring(MRM)mode using m/z 271.2>108.0 as quantitative ion pair and m/z 271.2>160.9 as qualitative ion pair.Quantitation was carried out using an external standard method.The detection limit of arbutin was 0.006mg/kg and the calibration curves were linear at the range of 0.004～0.2mg/L with linear correlation coefficient as 0.9994.The recoveries ranged from 80.6% to 108.1% with relative standard deviations less than 8.3%.The results of sample cleanup were well,and the determination method was simple,fast and sufficient.It was suitable for quantitative and qualitative analysis of arbutin in pomegranate juice concentrate.