中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 快生型大豆根瘤菌的理化特性和共生效应 大豆科学 1984 (2) 101-109
Position: Home > Articles > PHYSIOLOGICAL-BIOCHEMICAL CHARACTERISTICS AND SYMBIOTIC RESPONSE OF THE FAST-GROWING R. jappnicum Soybean Science 1984 (2) 101-109

快生型大豆根瘤菌的理化特性和共生效应

作  者：
徐玲玫;樊惠;葛诚;吴玉兰;陈月英;吴冈梵;张仁双;傅连舜
单  位：
中国农业科学院土壤肥料研究所
摘  要：
从辽宁分离的快生型大豆根瘤菌在柠檬酸盐培养基上表现出两种类型。12株中7株在柠檬酸盐培养基上不生长,5株生长良好。但生长速度比A.tumefaciens慢些。所有菌株都不产生3-酮基乳糖。在石蕊牛奶上除1株以外,都产生血清环,3株产酸,8株产碱1株不明显。在耐盐方面一般能耐0.3 M—0.4MNaCl,而能利用柠檬酸盐的菌株有4株能耐0.45 M Nacl的浓度。这些菌株和我国的栽培大豆品种铁丰18、开育8号、77—153等品种都能进行有效共生。但在干重、植株含氮量上均显著低于慢生型大豆根瘤菌。快型菌株和铁丰18、开育8号亲和性较好、77—153较差。111、121、123 A分别接在豇豆(北京红)和绿豆(D0245—1)、上,除111外其余两株都结瘤并有固氮酶活性。所有结瘤试验都用相应抗血清做了鉴定,证明确系由快生型大豆根瘤菌所结根瘤。另外一些理化特性鉴定正在进行。
译  名：
PHYSIOLOGICAL-BIOCHEMICAL CHARACTERISTICS AND SYMBIOTIC RESPONSE OF THE FAST-GROWING R. jappnicum
作  者：
Xu Lingmei Faa Hui Ge Cheng Wu Yulan Chen Yueueng(Soils and Fertilizers Research Institute, Chinses Academy of Agricultural Scineses)Wu Gangfan Zhang Renshuang Fu Lianshun(Tieling Institute of Agricultural Sciences)
摘  要：
There are two types of fast-growing Rhizobia isolated from nodules of wild Soybean(Glycine soja)growing in Liaoning Province on the Simmon's medium, in which 7 out of 12 strains didn't grow and the other 5 grew well, but their rates were slower than that of A, tumefaciens. All strains didn't produce 3-keto-1actose from lactose oxidation. Three strains showed acid reactions and eight strains showed alkaline reactions in litmus milk; but one strain (083) wasn't distinguishable. All fast-growing R. japonicum tested, except strain 066, formed serum ring. Twelve fast-growing R. iaponicum grew in concentrations of up to 0.3-0.4M NaGl, but four of them utilized citrate could tolerate concentrations of 0.45 M NaCl. These isolates formed effective nitrogen-fixing associations with Chinese soybean cuhivars such as Tiefeng 18, Kaiyu 8, 77-153 and so forth. However, dry weight and total nitrogen of above-ground portion were significantly lower than that of slow-growiag R.aponicum. The affinity between fast-growing rhizobia and Kaiyu 8, Tiefeng 18 was significant, but most of them didn't nodulate or formed ineffective nodules with soybean cultivar 77-153.Vigna unguculata and Phaseolus aureus inoculated with fast-growing rhizobia 111, 121,123A individually, except strain 111, other two strains (121, 123A) all produced nodules having activity of nitrogenase. All nodules of plants in pot culture test were identified with corresponding antisera. The result of our experiment showed that nodules of Glycine max, Vigna unguiculata, Phaseolus aureus were formed by fast-growing rhizobia.

相似文章

导出引用
计量
文章访问数: 5
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46