作 者：
龙栋磊;文江南;王敏;王荣;张秀敏;毛宏祥;邓近平;谭支良
单 位：
中国科学院亚热带农业生态研究所;湖南农业大学动物科学技术学院
关键词：
排气压力;体外发酵;气体生成;挥发性脂肪酸
摘 要：
本试验旨在研究不同排气压力对体外模拟瘤胃发酵特征的影响。选用3只体重相近、装有永久性瘤胃瘘管的成年湘东黑山羊作为瘤胃液供体动物,利用全自动体外模拟瘤胃发酵系统,选用6种发酵底物进行48 h体外发酵,研究9、14和19 k Pa 3种排气压力对总产气量、甲烷产量、产气参数、干物质消失率、发酵液p H以及挥发性脂肪酸产量等发酵参数的影响。结果表明:发酵底物对体外模拟瘤胃发酵参数影响显著(P<0.05);排气压力对体外模拟瘤胃发酵参数没有显著影响(P>0.05)。由此可见,排气压力由9 k Pa增至19 k Pa对体外模拟瘤胃发酵特征无影响。
译 名：
Automated Incubation System in Vitro: Venting Pressure Less Than 19 kPa did Not Affect Fermentation Characteristics
作 者：
LONG Donglei;WEN Jiangnan;WANG Min;WANG Rong;ZHANG Xiumin;MAO Hongxiang;DENG Jinping;TAN Zhiliang;College of Animal Science and Technology,Hunan Agricultural University;Hunan Co-Innovation Center of Animal Production Safety;Institute of Subtropical Agriculture ,Chinese Academy of Sciences;College of Animal Science ,South China Agricultural University;
单 位：
College of Animal Science and Technology,Hunan Agricultural University%Hunan Co-Innovation Center of Animal Production Safety%Institute of Subtropical Agriculture ,Chinese Academy of Sciences%College of Animal Science ,South China Agricultural University
关键词：
venting pressure;;in vitro fermentation;;gas production;;volatile fatty acids
摘 要：
The present study w as conducted to determine the effect of different venting pressure on in vitro fermentation characteristics. Rumen fluid w as collected from three adult Xiaongdong black goats w ith similar w eight and permanent rumen fistula,used automated incubation system in vitro,and six w idely varied substrates w ere selected for 48 h in vitro fermentation,and three venting pressures( 9,14 and 19 k Pa) w ere performed to investigate the effects of venting pressure on in vitro total gas production,methane production,in vitro dry matter disappearance,fermentation fluid p H and volatile fatty acid production et al. The results show ed that fermentation substrate had significant effects on in vitro rumen fermentation parameters( P< 0.05);how ever,venting pressure had no significant effects on in vitro fermentation parameters( P> 0.05). In conclusion,venting pressure increase from 9 k Pa to 19 k Pa do not affect fermentation characteristics.
