Position: Home > Articles > Effect of HOPX overexpression on expression of the important developmental genes in chicken adipose
Journal of Northeast Agricultural University
2015,46
(5)
44-50
过表达HOPX基因对鸡脂肪生长发育重要基因表达的影响
作 者：
王宁;史洪岩;程敏;孙婴宁;李辉
单 位：
农业部鸡遗传育种重点实验室东北农业大学动物科学技术学院
关键词：
HOPX基因;鸡;脂肪细胞分化;发育
摘 要：
为揭示HOPX基因在鸡脂肪生长发育中的作用,研究采用Real-time RT-PCR和启动子荧光素酶报告基因技术,分析过表达HOPX基因对鸡脂肪生长发育重要基因的表达及其启动子活性的影响。结果表明,与空载体对照组(p CMV-HA vector)相比,HOPX基因能够促进A-FABP和PPARγ基因表达及其启动子活性,抑制KLF 7基因表达及其启动子活性,但对FAS基因表达及其启动子活性没有影响。研究结果显示,过表达HOPX基因可能促进鸡前脂肪细胞分化。
译 名：
Effect of HOPX overexpression on expression of the important developmental genes in chicken adipose
作 者：
WANG Ning;SHI Hongyan;CHENG Min;SUN Yingning;LI Hui;Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, School of Animal Science and Technology, Northeast Agricultural University;
关键词：
HOPX;;chicken;;adipopocyte differentiation;;development
摘 要：
To explore the functions of HOPX in chicken adipose development, in the present study, the effects of HOPX overexpression on mR NA expression and promoter activity of the important developmental genes in chicken adipose tissue were tested using Real-time RT-PCR and luciferase reporter assays. The results showed that compared to empty vector control, HOPX overexpression increased the mR NA expression and promoter activities of A- FABP and PPARγ genes, decreased the mR NA expression and promoter activity of KLF 7 gene, but HOPX overexpression had no effect on mR NA expression and promoter activity of FAS gene. The results of this study indicate that HOPX may promote chicken preadipocyte differentiation.
导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
