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当前位置: 首页 > 文章 > 绿僵菌野生菌株对红脉穗螟幼虫的致病效果研究 江西农业大学学报 2014 (6) 1253-1257
Position: Home > Articles > Bioassay of Toxicity of Metarhizium anisopliae Wild Strains to Larvae of Tirathaba Rufivena Acta Agriculturae Universitatis Jiangxiensis 2014 (6) 1253-1257

绿僵菌野生菌株对红脉穗螟幼虫的致病效果研究

作  者：
吕朝军;钟宝珠;钱军;苟志辉;孙晓东;覃伟权
单  位：
中国热带农业科学院椰子研究所;海南省林业科学研究所
关键词：
绿僵菌;红脉穗螟;致病力;半致死时间(LT50);僵虫率
摘  要：
采用孢子悬浮液喷雾法测定不同绿僵菌(Metarhizium anisopliae)野生菌株对红脉穗螟(Tirathaba rufivena)幼虫的致病能力,旨在获得对该虫具有高致病力的生防菌株,以期为红脉穗螟的生物防治提供新的生物资源。结果表明,G2和G3菌株对红脉穗螟幼虫表现出较高致病力,在1.0×108孢子/m L浓度下,两个菌株对红脉穗螟幼虫的累计校正死亡率分别为86.32%和80.34%,LT50分别为4.77 d、6.18 d;田间试验表明,G2和G3菌株在1.0×1010孢子/m L浓度下,药后7 d可使红脉穗螟的虫口减退率分别达到73.72%和68.71%,表明这两株菌株在红脉穗螟生物防治中是极具有应用潜力的生防真菌。
译  名：
Bioassay of Toxicity of Metarhizium anisopliae Wild Strains to Larvae of Tirathaba Rufivena
作  者：
LYU Chao-jun;ZHONG Bao-zhu;QIAN Jun;GOU Zhi-hui;SUN Xiao-dong;QIN Wei-quan;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences;Forestry Research Institute of Hainan Province;
关键词：
Metarhizium anisopliae;;Tirathaba rufivena;;pathogenicity;;median lethal time(LT50);;cadaver rate
摘  要：
In order to screen entomopathogenic fungi strains with high virulence against the larva of Tirathaba rufivena,the evaluation of the pathogenicity of Metarhizium anisopliae to T.rufivena larvae was conducted using the spore suspension spray method. The results indicated that trains of G2 and G3 had higher virulence to T.rufivena larvae,and the cumulative mortality rates were 86.32% and 80.34%,respectively; the median lethal times( LT50) were 4.77 d and 6.18 d at the concentration of 1.0×108spores·m L-1.The field trial results showed that the pest population declined by 73.72% and 68.71% after treated with G2 and G3 at the concentration of 1.0×108spores·m L-17 d after the treatment.The results suggest that G2 and G3 M.anisopliae strains have potential in biocontrol of T.rufivena.

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