作 者：
程静;张癸新;陈厚桦;杨森;李培源;陈静仪;梁日深
关键词：
胡椒鲷;系统进化;线粒体DNA;核DNA
摘 要：
为从分子水平分析胡椒鲷属鱼类分子系统分类关系,澄清物种分类争议,采集印度-西太平洋分布的胡椒鲷鱼类 16 种,利用PCR扩增及测序方法获得这 16 种鱼类线粒体 Cyt b 及核 RAG2、S7 标记序列,基于最大似然法与贝叶斯法构建分子系统进化关系.结果显示:16 种鱼类 Cyt b 同源序列为657 bp,编码 219 个氨基酸;RAG2 同源序列为 726 bp,编码 243 个氨基酸;S7 基因同源序列为 729 bp,序列存在大量碱基插入与缺失.以大斑石鲈及断斑石鲈为外类群构建分子系统进化树,进化树上胡椒鲷鱼类主要分为 3 个分支,分支Ⅰ胡椒鲷具鲜艳的体色与斑纹,分支Ⅱ与分支Ⅲ胡椒鲷色彩单一,无鲜艳斑纹,与形态分类结果相似.少棘胡椒鲷属在进化树上并未形成独立的分支,而是位于胡椒鲷属内部.比较分析少棘胡椒鲷属、胡椒鲷属鱼类鳍棘数目与各物种在进化树上的分类关系,并未发现鳍棘数目与物种亲缘关系存在相关性.研究结果表明,在胡椒鲷类群中,背鳍鳍棘数可作为形态上区分不同胡椒鲷种类的指标,但未必能作为胡椒鲷鱼类亲缘关系远近的划分依据.结果支持目前大多数分子分类研究结果,少棘胡椒鲷鱼类应归为胡椒鲷属.
译 名：
Molecular Phylogenetic Relationships among 16 Species of Sweetlips Based on 3 Gene Sequences
关键词：
sweetlip%phylogeny%mitochondrial DNA%nuclear DNA
摘 要：
A total of 16 species of sweetlips were obtained,14 species belonged to the genus Plectorhin-chus,2 species belonged to the genus Diagramma,domestic samples from the South China Sea and sur-rounding seas,while most of the foreign samples from the Indian Ocean,one in the South Pacific,and the mitochondrial Cyt b and nuclear RAG2 and S7 labeling sequences of these 16 fish species were obtained by PCR amplification and sequencing.The phylogenetic relationship of analytical molecules was constructed based on maximum likelihood method and Bayesian method.The results showed that the homologous se-quence of 16 sweetlips in Cyt b genes were determined to be 657 bp,encoding 219 amino acids;the homol-ogous sequences in RAG2 gene were 726 bp,encoding 243 amino acids,the homologous sequence in S7 genes were 729 bp,with numerous base insertions and deletions.The molecular phylogenetic tree were constructed using Pomadasys maculatus and P.hasta as outgroup species,sweetlips in the tree were di-vided into three major groups.Species in group Ⅰ possessed spectacular coloration and markings along bodies while species in group Ⅱ and group Ⅲ had monotone color and uniformly dark patterns,which were consistent with the morphological classification.Genus Diagramma did not form an independent branch but were clustered inside the Plectorhinchus group.Comparison of the dorsal spine numbers of Di-agramma and Plectorhinchus with their relationships in the phylogenetic tree revealed that no correlation was found between dorsal spine numbers and species phylogenetic relationship,indicating that numbers of dorsal spines was used as an indicator to distinguish different sweetlips morphologically,but not be used as a basis to investigate the their relationships.The findings supported the viewpoints of most current molec-ular phylogenetic studies that genus Diagramma was classified into genus Plectorhinchus.
