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当前位置: 首页 > 文章 > CHA结合DNAzyme用于沙门氏菌核酸的可视化检测 东北农业科学 2017 (3) 44-49
Position: Home > Articles > Visual Detection of Salmonella DNA Fragments by CHA Combined DNAzyme Journal of Northeast Agricultural Sciences 2017 (3) 44-49

CHA结合DNAzyme用于沙门氏菌核酸的可视化检测

作  者：
卜胜君;王奎宇;宋广萍;英娜;齐炳尧;李忠义;汤鑫;万家余;张红梅
单  位：
黑龙江八一农垦大学;军事医学科学院军事兽医研究所
关键词：
沙门氏菌;特异性;16s rRNA;CHA;DNAzyme;可视化检测
摘  要：
为建立简洁、灵敏、快速的食源性沙门氏菌核酸可视化检测方法,试验采用靶标催化发夹装置(Catalyzed hairpin assembly,CHA)结合功能性核酸DNAzyme的方法,设计沙门氏菌核酸发夹型抓取探针(Capture probe,CP),CP结合靶标并启动H1、H2引发CHA反应,形成DNAzyme结构并发生显色反应。试验中对CP、H1、H2最佳浓度及缓冲液pH进行优化,对灵敏度与特异性进行分析。结果表明:该方法对沙门氏菌核酸片段的可视化检测具有高特异性,灵敏度达到3.9pM,且存在一定的线性关系。说明此方法的建立可为食源性病菌可视化检测奠定相应基础。
译  名：
Visual Detection of Salmonella DNA Fragments by CHA Combined DNAzyme
作  者：
BU Shengjun;WANG Kuiyu;SONG Guangping;YING Na;QI Bingyao;LI Zhongyi;TANG Xin;WAN Jiayu;ZHANG Hongmei;Heilongjiang Bayi Agricultural Reclamation University;Institute of Military Veterinary,Academy of Military Medical Sciences;
关键词：
Salmonella;;Specificity;;16s rRNA;;CHA;;DNAzyme;;Visual detection
摘  要：
A target catalyzed hairpin assembly combined with DNAzyme was adopted to establish a simple and sen-sitive method for visual detection of salmonella specific DNA fragment. Capture probe was designed by specific sal-monella DNA segments. When target was present, capture probe opened and started the CHA reaction. H1-H2 dip-loid DNAzyme structure was formed and caused chromogenic reaction. The experiment was carried out to select opti-mal content of CP, H1, H2, and pH of buffer solution, and analyze the sensitivity and specificity. The results showedthat the system had a good generality and met the requirements of the naked eye visual detection. The detect limit ofthis method was 3.9 pM and there was a linear correlation. Setting up of this method laid basis for visual detection offood-borne bacteria.

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