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当前位置: 首页 > 文章 > 杜仲橡胶合成相关酶基因HMGR的克隆与表达分析 植物遗传资源学报 2016,17 (4) 726-731
Position: Home > Articles > Cloning and Expression Analysis of HMGR Involved in Rubber Biosynthesis of Eucommia ulmoides Oliv. Journal of Plant Genetic Resources 2016,17 (4) 726-731

杜仲橡胶合成相关酶基因HMGR的克隆与表达分析

作  者：
荆腾;王淋;乌云塔娜;杜红岩
单  位：
中国林业科学研究院经济林研究开发中心/国家林业局杜仲工程技术研究中心
关键词：
杜仲;3-羟基-3-甲基戊二酰辅酶A还原酶;基因表达;杜仲橡胶
摘  要：
为解析HMGR基因在杜仲橡胶生物合成中的作用,本研究以杜仲良种华仲6号叶片和果皮为材料,参考杜仲基因组和转录组数据,克隆了杜仲HMGR基因,命名为Eu HMGR,全长1770 bp,编码590个氨基酸。实时荧光定量PCR测定结果显示,EuHMGR基因在果皮中的表达量于5月中旬达到最大值,而在叶片中的最大值出现在7月中旬。通过索氏提取测定相应时期果皮和叶片的含胶量表明,果皮中的含胶量从4月中旬到5月下旬迅速增加,而叶片中的含胶量一直平稳增长。分析不同发育时期果皮和叶片Eu HMGR基因表达量和含胶增长速率相关性发现,果皮Eu HMGR基因在不同发育时期的相对表达量和含胶增长速率呈显著正相关,而二者在叶片中无明显相关性。因此,推测Eu HMGR基因的表达与杜仲果皮中橡胶生物合成相关。
译  名：
Cloning and Expression Analysis of HMGR Involved in Rubber Biosynthesis of Eucommia ulmoides Oliv.
作  者：
JING Teng;WANG Lin;WUYUN Ta-na;DU Hong-yan;Non-timber Forest Research and Development Center of Chinese Academy of Forestry,The Eucommia Engineering Research Center of State Forestry Administration;
关键词：
Eucommia ulmoides Oliv;;3-hydro-3-methylglutary Co A reductase;;gene expression;;Eucommia ulmoides Oliv
摘  要：
In order to investigate the characteristic of HMGR gene associated with the rubber biosynthesis in Eucommia ulmoides Oliv.,based on sequences of Eucommia ulmoides Oliv. genome and transcriptomedata,a 1770 bp full-length c DNA encoding 590 amino acid residues,named as Eu HMGR,was cloned by using leaves and pericarp of Huazhong No. 6 as the template,respectively. Quantitative real-time PCR( qRT-PCR) experiments showed that Eu HMGR mRNA accumulation in pericarp was the highest in mid-May,nevertheless,that in leaves was in the middle of July. Moreover,the content of rubber in leaves and pericarp was determined by Soxhlet extraction at each corresponding time point. The result showed that the content of rubber increased rapidly in pericarp from mid-April to late-May compared with the steady growth in leaves in all tested time points. Besides,the correlation analysis suggested that there was a significant correlation between Eu HMGR expression patterns and accumulation rate of EuRubber in leaves and pericarps at different developmental stages,and that not in leaves,which speculated that the expression of Eu HMGR was related with the biosynthesis of rubber.

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