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Position: Home > Articles > Association Analysis of Crude Fat and Fatty Acid Components in Flax Based on SSR Markers Crops 2022 (1) 44-49

基于SSR标记的胡麻粗脂肪及脂肪酸组分的关联分析

作  者：
高凤云;斯钦巴特尔;周宇;贾霄云;苏少锋;赵小庆;金晓蕾
单  位：
内蒙古自治区农牧业科学院特色作物研究所/内蒙古自治区农牧业科学院生物技术研究所
关键词：
胡麻;品质;粗脂肪;脂肪酸组分;关联分析;SSR
摘  要：
为了深入了解胡麻种质粗脂肪及脂肪酸组分的遗传变异,以230份胡麻种质为研究对象,利用广义线性模型(GLM)进行关联分析,结果表明,粗脂肪和5种脂肪酸含量的变异系数为7.39%～22.67%,遗传多样性指数为2.66～2.80;粗脂肪和亚麻酸含量的相关系数(0.669)最大;筛选出了30对SSR引物,共扩增出365个条带,有效等位基因数在1.2168～1.8320,引物多态性含量为0.2489～0.6257;在群体结构K=4时,230份胡麻资源可分为4个类群;5种脂肪酸显著关联的SSR位点22个,粗脂肪含量相关的SSR位点4个,说明230份胡麻种质的遗传多样性丰富,5种脂肪酸和粗脂肪含量与SSR标记显著关联。
译  名：
Association Analysis of Crude Fat and Fatty Acid Components in Flax Based on SSR Markers
作  者：
Gao Fengyun;Siqin Bateer;Zhou Yu;Jia Xiaoyun;Su Shaofeng;Zhao Xiaoqing;Jin Xiaolei;Institute of Characteristic Crops, Inner Mongolia Academy of Agriculture and Husbandry Sciences/Institute of Biotechnology, Inner Mongolia Academy of Agriculture and Husbandry Sciences;
关键词：
Flax;;Quality;;Crude fat;;Fatty acid components;;Correlation analysis;;SSR
摘  要：
In order to understand the genetic variation of crude fat and fatty acid components in flax germplasm,the generalized linear model(GLM) was used to analyze the genetic variation of 230 flax germplasms. The results showed that the variation coefficients of crude fat and five fatty acids were 7.39%-22.67%, and the genetic diversity indexes were 2.66-2.80. The correlation coefficient between crude fat and linolenic acid was the highest(0.669).Thirty pairs of SSR primers were selected, and a total of 365 bands were amplified. The number of effective alleles ranged from 1.2168 to 1.8320, and the polymorphism of primers ranged from 0.2489 to 0.6257. The 230 flax resources could be divided into four groups when population structure K=4. There were 22 SSR loci significantly associated with five fatty acids, and four SSR loci were detected in crude fat. These results indicated that the genetic diversity of 230 flax germplasms was rich, and the contents of five fatty acids and crude fat were significantly associated with SSR markers.

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