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Position: Home > Articles > ERIC- PCR fingerprints of Haemophilus parasuis strains isolated from Jiangxi during 2012 and 2014 Animal Husbandry & Veterinary Medicine 2015,47 (6) 36-40

2012~2014年江西地区副猪嗜血杆菌分离株ERIC-PCR指纹图谱分析

作  者:
曾文斌;刘悦欣;熊亚坤;左明开;幸文定;王萍
单  位:
江西农业大学动物科学技术学院;九江市畜牧兽医局
关键词:
副猪嗜血杆菌;ERIC-PCR;指纹图谱;基因分型
摘  要:
在对15种副猪嗜血杆菌血清型参考株鉴定获得15种不同ERIC-PCR指纹的基础上,对2012~2014年分离自江西地区41株副猪嗜血杆菌临床分离菌株进行指纹鉴定。结果表明,41株副猪嗜血杆菌产生20种不同的指纹图谱,相同血清型的菌株表现出不同的指纹图谱,无法进行血清分型的副猪嗜血杆菌应用该方法可得到充分区分。该方法证实副猪嗜血杆菌ERIC-PCR指纹图谱存在丰富的多样性,可适用于副猪嗜血杆菌的快速基因分型及分子流行病学调查。
译  名:
ERIC- PCR fingerprints of Haemophilus parasuis strains isolated from Jiangxi during 2012 and 2014
作  者:
ZENG Wenbin;LIU Yuexin;XIONG Yakun;ZUO Mingkai;XING Wending;WANG Ping;College of Animal Science and Technology,Jiangxi Agricultural University;Animal Husbandry and Veterinary Bureau of Jiujiang;
关键词:
Haemophilus parasuis;;ERIC-PCR;;fingerprint;;genotyping
摘  要:
Based on the identification of ERIC- PCR fingerprints for 15 reference strains of Haemophilus parasuis from different serotypes,a total of 41 strains isolated from Jiangxi during 2012 to 2014 were analyzed by ERIC- PCR. The results showed that 20 different ERIC-PCR profiles were observed among the isolates. And different serotypes had distinct fingerprints,but so did varieties within a serotype. The isolates which could not be serotyped could be genotyped by ERIC- PCR. It suggests that ERIC- PCR DNA fingerprinting technique used in this study is an effective way for molecular epidemiological investigation of H. Parasuis.

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