Establishing and Screening the cDNA Libraries of Adult and Newborn Larvae of Chinese Trichinella isolates

Liu Mingyuan Xu Kecheng Li Chengyao Liu Zengshan Sun Yuantao Zhao Jun Zheng Mingguang (Trichinellosis Lab, Uni of Agri and Anim Sci, Changchun 130062) Boireau P Fabien J F Trap C Vayssier M Vallet C Perret C Soule C (Molecular Biology La

cDNA libarary; adult and newborn larvae; Trichinella

In order to find stage spectific genes and immunodominant antigens, the cDNA libraries of adult and newborn larvae of the two Chinese Trichinella isolates were established and screened with λZAPⅡ vector and random amplified polymorphic DNA (RAPD) technology. The result showed that all cloned cDNA fragments were between 0.2 2.0 kb, suggesting the two cDNA libraries would have covered its almost all mRNA. Using 70 single primers and 20 multiplex primers to screen the cDNA libraries by RAPD technology, 2 T.spiralis and 4 T.nativa adult and newborn stage specific genes and 1 specific gene of T.spiralis obtained, T.spiralis and T. nativa having 2 common stage specific genes. The stage specific genes would be used to select stage specific cDNA clones from recombinant cDNA libraries, and to screen immunodominant antigenic genes for constructing recombinant vaccine of Trichinella. The species specific gene could be used for the species identification of Trichinella isolates. Using RAPD technology for screening cDNA libraries, there were almost no differences between different country isolates (Chinese and French T.spiralis isolates) on the amplified patterns except amplified efficient rate (bright or dark) in certain bands, indicating that there have been a very low gene flow within the species of Trichinella.