译 名：
Study on Quality Standard of Chinese Veterinary Medicine Wumei Granule
作 者：
ZHANG Yuan-da;CHEN Zhi-qiang;SHANG Jing-xue;CAI Xiao-li;ZHAN Wen;ZHANG Peng-ju;FU Li-yun;LI Hai-hua;Inner Mongolia huatian pharmaceutical co.,LTD.;
关键词：
Wumei Granules;;Citrate;;thin layer chromatography;;high performance liquid chromatography
摘 要：
To establish the quality standard of Wumei Granules. Thin layer chromatography( TLC) was used to identify the black plum and coptis chinensis. The content of citric acid in wumei granules was determined by HPLC. The chromatographic condition was Silgreen C18( 4.6 mm × 250 mm,5 μm) column. The mobile phase was acetonitrile-0.5% ammonium dihydrogen phosphate solution( 3 ∶97)( p H value adjusted by phosphoric acid to 3.0). The detection wavelength was 210 nm. The flow rate was 0.6 m L/min. The column temperature is room temperature. A TLC method for the qualitative identification of fructus mume and rhizoma coptidis was established.HPLC method for determination of citric acid in wumei gran ules was established. The method is simple,easy to operate,accurate and reproducible. It can be used for quality control of Wumei Granules.
