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Position: Home > Articles > Magnetic immunochromatography assay for rapid detection of Cucumber green mottle mosaic virus Plant Protection 2014,40 (3) 113-116

黄瓜绿斑驳花叶病毒磁免疫层析试纸条的研制

作  者:
魏梅生;李桂芬;马洁;朱水芳;王军平;文朝慧
单  位:
甘肃出入境检验检疫局;中国检验检疫科学研究院植物检疫研究所
关键词:
黄瓜绿斑驳花叶病毒;磁免疫层析;试纸条
摘  要:
用磁性纳米颗粒标记黄瓜绿斑驳花叶病毒的兔多克隆抗体建立了简便快速的磁免疫层析试纸条检测方法。该方法检测提纯病毒的灵敏度为0.1μg/mL,检测葫芦病汁液可稀释10 000倍(重量/体积)。试纸条和黄瓜绿斑驳花叶病毒具有特异性反应,和同属的烟草花叶病毒、番茄花叶病毒、辣椒轻斑驳病毒和齿兰环斑病毒,未出现交叉反应。该试纸条可用于田间病害检测和诊断。
译  名:
Magnetic immunochromatography assay for rapid detection of Cucumber green mottle mosaic virus
作  者:
Wei Meisheng;Li Guifen;Ma Jie;Zhu Shuifang;Wang Junping;Wen Zhaohui;Institute of Plant Quarantine,CAIQ;Gansu Entry-Exit Inspection and Quarantine Bureau;
关键词:
Cucumber green mottle mosaic virus;;magnetic immunochromatography assay;;immunostrip
摘  要:
Rapid magnetic immunochromatography assay was developed by coupling magnetic nanoparticles with polyclonal rabbit antibody against Cucumber green mottle mosaic virus(CGMMV).Sensitivity of the method was 0.1μg/mL for detecting purified CGMMV.CGMMV could be detected in the extract of the infected Lagenaria sicerarialeaves at a dilution of 10-4(W/V).The immunostrip was specific to CGMMV,without cross reaction with other tobamoviruses such as Tobacco mosaic virus,Tomato mosaic virus,Pepper mild mottle virus and Odontoglossum ringspot virus.The immunostrip is an ideal screening tool for field inspection and disease diagnosis.

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