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Position: Home > Articles > High Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for the Analysis of Anthocyanins in Blackberry Fruit FOOD SCIENCE 2009,30 (14) 230-234

高效液相色谱-串联质谱法分离鉴定黑莓花色苷

作  者:
王卫东;李超;许时婴
单  位:
徐州工程学院食品工程学院;江南大学食品学院
关键词:
黑莓;花色苷;高效液相色谱-串连质谱(HPLC/ESI-MS/MS)
摘  要:
利用高效液相色谱与二极管阵列检测器/电喷雾质谱联用技术研究了黑莓中的花色苷类化合物,分离鉴定出四种花色苷,均为矢车菊素花色苷。除了一种花色苷是由阿拉伯糖和矢车菊合成苷外,其他花色苷的糖基均为葡萄糖,并且有两种花色苷和脂肪酸发生酰化。矢车菊3-O-葡萄糖苷是黑莓中的主要花色苷,占全部色素含量的76.92%,矢车菊3-O-阿拉伯糖苷、矢车菊3-O-丙二酸酰葡萄糖苷和矢车菊3-O-草酸酐酰葡萄糖苷含量依次为5.57%、2.76%和14.75%。
译  名:
High Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for the Analysis of Anthocyanins in Blackberry Fruit
作  者:
WANG Wei-dong1,2,LI Chao1,XU Shi-ying2,* (1. College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China; 2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
关键词:
blackberry;anthocyanin;high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS)
摘  要:
The anthocyanin compositions in blackberry fruit were isolated using high performance liquid chromatography (HPLC) equipped with a lichrospher C18 column and a DAD detector and characterized by UV spectrometry, gas chromatography (GC) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). A total of 4 anthocyanins were found to be isolated on the C18 column. Among them were cyanidin (Cy) 3-O-glucoside (76.92%) , Cy 3-O-arabinoside (5.57%), Cy 3-O- malonylglucoside(2.76%)and Cy 3-O-dioxalylglucoside (14.75%).

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