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当前位置: 首页 > 文章 > 小麦全蚀病菌拮抗菌Bacillus methylotrophicusZ-9菌株产芽孢条件优化 河南农业科学 2016,45 (1) 88-91
Position: Home > Articles > Conditions Optimization for Spore Production of Antagonistic Bacterium Bacillus methylotrophicus Z-9 against Wheat Take-all Disease Journal of Henan Agricultural Sciences 2016,45 (1) 88-91

小麦全蚀病菌拮抗菌Bacillus methylotrophicusZ-9菌株产芽孢条件优化

作  者：
孔少元;吕纪涛;张冬冬;王世英
单  位：
河北农业大学生命科学学院
关键词：
小麦全蚀病;拮抗菌;芽孢杆菌;芽孢;发酵条件;优化;芽孢产率
摘  要：
甲基营养型芽孢杆菌(Bacillus methylotrophicus)Z-9菌株对小麦全蚀病病原菌有显著拮抗活性,以芽孢产率和活菌数为主要指标,采用单因素试验和正交试验对Z-9菌株摇瓶产芽孢条件进行优化,为其工业生产提供试验依据。通过单因素试验确定最佳碳源、氮源和无机盐分别为玉米粉、黄豆饼粉和MnSO_4·H_2O、Mg SO_4·7H_2O。通过正交试验确定最适培养基组分为玉米粉1.0%、黄豆饼粉2.0%、MnSO_4·H_2O 0.04%、MgSO_4·7H_2O 0.04%、Na_-2HPO_4·2H_2O 0.4%、Na H_2PO_4·2H2O 0.2%;最适发酵条件为:初始p H值7.5,250 m L三角瓶装液量50 m L,37℃培养36 h。优化后发酵液的芽孢数量为1.60×109cfu/m L,较优化前提高了13.7倍,芽孢产率达到96.5%。
译  名：
Conditions Optimization for Spore Production of Antagonistic Bacterium Bacillus methylotrophicus Z-9 against Wheat Take-all Disease
作  者：
KONG Shaoyuan;Lü Jitao;ZHANG Dongdong;WANG Shiying;College of Life Science,Agriculture University of Hebei;
关键词：
wheat take-all disease;;antagonistic bacterium;;Bacillus;;spore;;fermentation conditions;;optimization;;spore production rate
摘  要：
Significant antagonistic activity of strain Bacillus methylotrophicus Z-9 against wheat take-all disease was shown. In order to optimize spore production conditions of the strain Z-9,researches were done by single-factor tests and orthogonal tests with number of viable cells and spore production rate as indexes. The best carbon source,nitrogen source and inorganic salt were corn flour,soybean meal,and Mn SO_4· H_2O,MgSO_4·7H_2O,respectively,by the single factor experiments. The optimum medium components were determined as corn flour 1. 0%,soybean meal 2. 0%,Mn SO_4·H_2O 0. 04%,Mg SO_4·7H_2O 0. 04%,Na_2HPO_4· 2H_2 O 0. 4%,N H_2PO_4· 2H_2 O 0. 2%,and the optimum fermentation conditions were determined as initial p H value of 7. 5,medium volume of 50 m L in 250 m L flask,cultivating at 37 ℃ for 36 h by the orthogonal experiments. The spore yield reached 1. 60 × 109 cfu / m L,which was improved by 13. 7 times,compared to the result before optimization,and the spore production rate reached 96. 5% under the optimum conditions.

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