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Sequence Analysis on Interleukin-2 Gene and Interleukin-6 Gene in a Hybrid Pig

作者：

Zhiqiang Shen;Ming Wang;Deming Zhao;Jinliang Wang;Na Tang;Gui‐Rong Q

单位：

Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, Chin;Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China

关键词：

pig;l-2;il;100;il-6 gene;9

摘要：

[Objective] To understand the functions of interleukin-2 (IL-2) and interleukin-6 (IL-6) in immune response. [Method] Peripheral lymphocytes were isolated from a hybrid pig (Duroc×Landrace×Shandong local pig) and stimulated with canavalin A in vitro. With total RNA as templates, the IL-2 and IL-6 cDNA were amplified by the RT-PCR and sequenced, respectively. [Result] The sequence of IL-2 gene had 100.0% nucleotide homology to the published IL-2 sequences. The sequence of IL-6 gene had 99.8%-100.0% nucleotide homology to the published IL-6 sequences, and the amino acid homology was 99.1%-100.0%. [Conclusion] No great variation of the IL-2 gene and IL-6 gene is observed in the hybrid pig, and these results provide a basis for research about functions of IL-2 and IL-6 protein.

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Sequence Analysis on Interleukin-2 Gene and Interleukin-6 Gene in a Hybrid Pig

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