当前位置: 首页 > 文章 > 利用激光共聚焦显微镜研究孝顺竹花粉粒发育 林业科学 2010,46 (11) 158-161+195
Position: Home > Articles > Observation of Pollen Development in Bambusa multiplex with Laser Scan Confocal Microscope Scientia Silvae Sinicae 2010,46 (11) 158-161+195

利用激光共聚焦显微镜研究孝顺竹花粉粒发育

作  者:
徐川梅;高欣;汤定钦
单  位:
浙江农林大学亚热带森林培育国家重点实验室培育基地
关键词:
孝顺竹;花粉粒发育;激光扫描共聚焦显微镜
摘  要:
激光扫描共聚焦显微镜(laser scanning confocal microscope,LSCM)是20世纪80年代发展的高科技产品,主要是采用激光作为光源,在荧光显微镜的基础上添加了激光扫描装置和计算机图像处理系统等,通过对观察样品进行光学断层扫描及三维结构重建等,可得到细胞或组织内部细微结构的荧光图像,同时激光扫描共聚焦显微镜还是活细胞动态观察、多重免疫荧光标记和Ca2+荧光观察(Chen et al.,2009)的有力工具,目前已在花粉(Fang et al.,2008)和花粉管发育(Chen et al.,2008;Wu et al.,2008)、蛋白质功能(Zheng et al.,2009)及信号传导(Liu et al.,2009)等的
译  名:
Observation of Pollen Development in Bambusa multiplex with Laser Scan Confocal Microscope
作  者:
Xu Chuanmei Gao Xing Tang Dingqin(The Nurturing Station for the State Key Laboratory of Subtropical Silviculture,Zhejiang Agriculture and Forestry University Lin'an 311300)
关键词:
Bambusa multiplex;development of pollen;laser scanning confocal microscope
摘  要:
The cytol development of male gametophyte of Bambusa multiplex was studied by dying with a fluorescence dye of Hoechst 33342 and observing with laser scanning confocal microscope(LSCM).The result showed twice mitoses took place and the mature pollens had three cells with one aperture during the development of the male gametophytes,and the male gametophytes abnormally acted at a frequency of 38% in B.multiplex.With the confocal arrangement,serial optical scanning,and advanced three-dimensional reconstruction of the LSCM,direct and visual display of the three-dimensional pollen architecture was available in this study.

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