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Position: Home > Articles > Analysis of Citrinin in Hongqu Product by ELISA FOOD SCIENCE 2004,25 (8) 169-172

红曲产品中桔霉素含量的ELISA测定

作  者:
陈福生;邢淑婕
单  位:
华中农业大学食品科技学院
关键词:
红曲;桔霉素;酶联免疫吸附测定
摘  要:
制备了牛血清白蛋白——桔霉素包被抗原和卵蛋白——桔霉素免疫抗原,用卵蛋白——桔霉素连接物免疫小白鼠,间接ELISA测小鼠抗血清效价,待血清效价达到要求后,腹腔注射骨髓瘤细胞,10~15d后抽取腹水离心得抗体。建立竞争ELISA检测红曲样品桔霉素含量的方法,研究了该法的特异性和桔霉素的加标回收,结果表明,此法具有较高的特异性和加标回收能力,桔霉素的测定浓度范围为5.0~200ng/ml,回收率97%~104%。
译  名:
Analysis of Citrinin in Hongqu Product by ELISA
作  者:
CHEN Fu-sheng,XING Shu-jie(College of Food science and Technology, Huazhong Agricultural University,Wuhan 430070, China)
关键词:
Hongqu;citrinin;enzyme-linked immunosorbent assay
摘  要:
The immune antigen of OV-citrinin and the coated antigen of BSA-citrinin were synthesized respectively andidentified with ultraviolet absorption spectrometry. The competitive ELISA for detecting the citrinin content in the Hongquproduct was developed, with the detecting concentration range 5.0~200ng/ml of citrinin. The recovery of this method is 97%~104%.

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