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Position: Home > Articles > Determination on Magnolol and Honokiol Contents in Huoxiangzhengqi Tincture by RP-HPLC Method Journal of Anhui Agricultural Sciences 2007,35 (29) 36+55

RP-HPLC法测定藿香正气水中厚朴酚与和厚朴酚的含量

作  者:
刘晓鹏;姜宁
单  位:
湖北民族学院生物科学与技术学院;生物资源保护与利用湖北省重点实验室
关键词:
厚朴酚;和厚朴酚;藿香正气水;RP-HPLC;含量
摘  要:
[目的]测定藿香正气水中厚朴酚及和厚朴酚的含量。[方法]利用反相高效液相色谱法测定藿香正气水中厚朴酚及和厚朴酚的含量,用Diamonsil(TM)C18柱(250 mm×4.6mm,5μm),以甲醇∶水∶冰醋酸(79:21:0.25)为流动相,流速为1.0ml/min,检测波长为294nm,柱温为23℃。[结果]RP-HPLC法测得厚朴酚与和厚朴酚浓度在6.25~400μg/ml范围内与峰面积线性关系良好。和厚朴酚的平均回收率为98.4%,RSD为1.58%;厚朴酚的平均回收率为100.35%,RSD为1.24%。[结论]该方法简便、准确、专属性强,能有效地控制藿香正气水的检测质量。
译  名:
Determination on Magnolol and Honokiol Contents in Huoxiangzhengqi Tincture by RP-HPLC Method
作  者:
LIU Xiao-peng et al(College of Biological Sciences and Technology,Hubei Institute for Nationalities,Enshi,Hubei 445000)
关键词:
Magnolol;Honokiol;Huoxiangzhengqi tincture;RP-HPLC;Contents
摘  要:
[Objective] The aim of the study was to determine magnolol and honokiol contents in Huoxiangzhengqi tincture.[Method] With Diamonsil(TM)C18 column(250 mm × 4.6 mm,5 μm),methanol-water-acetic acid(79∶21∶0.25) as mobile phase,flow velocity at 1.0 mL/min,detection wave length at 294 nm and column temperature at 23 ℃,the magnolol and honokiol contents in Huoxiangzhengqi water were determined by RP-HPLC.[Result] The magnolol and honokiol contents determined by RP-HPLC method had good linear relationship with the peak areas when their concentrations were at 6.25~400 μg/mL.The average recoveries and RSDs of magnolol were 100.78 % and 99.6 % resp.and those of honokiol were 1.52 % and 1.18 % resp.[Conclusion] This method was simple and convenient,accurate and specific,which could control the checking quality of Huoxiangzhengqi tincture effectively.

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