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当前位置: 首页 > 文章 > 椰子织蛾高毒力金龟子绿僵菌菌株的筛选 植物保护 2016,42 (6) 215-218
Position: Home > Articles > Screening of highly toxic strains of Metarhizium anisopliae against Opisina arenosella Walker Plant Protection 2016,42 (6) 215-218

椰子织蛾高毒力金龟子绿僵菌菌株的筛选

作  者：
孙晓东;李富恒;阎伟;李朝绪;吕朝军;覃伟权
单  位：
东北农业大学生命科学学院;中国热带农业科学院椰子研究所
关键词：
金龟子绿僵菌;椰子织蛾;分子鉴定;毒力
摘  要：
椰子织蛾是为害热带地区棕榈科植物的重要害虫,本试验对从海南省陵水县采集的椰子织蛾僵虫上分离得到的4株金龟子绿僵菌进行了分子生物学鉴定,并测定了其对椰子织蛾3龄幼虫的室内生物活性,筛选出了高毒力菌株LS-Y1。试验结果表明,经NCBI BLAST比对,4株菌株的rDNA-ITS序列与GenBank中已知基因序列相似性分别为99%、100%、99%、99%。菌株LS-Y1处理椰子织蛾3龄幼虫后6d的LC50为4.41×106个/mL。6.25×106、1.25×107、2.5×107、5×107和1×108个/mL浓度处理下,金龟子绿僵菌LS-Y1对椰子织蛾3龄幼虫的LT50分别为5.77、4.88、4.43、3.82和3.36d。该菌株对椰子织蛾幼虫具有较高毒力,具有较好的生防潜力。
译  名：
Screening of highly toxic strains of Metarhizium anisopliae against Opisina arenosella Walker
作  者：
Sun Xiaodong;Li Fuheng;Yan Wei;Li Chaoxu;LüChaojun;Qin Weiquan;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences;College of Science,Northeast Agricultural University;
关键词：
Metarhizium anisopliae;;Opisina arenosella;;molecular identification;;toxicity
摘  要：
Opisina arenosella Walker is an important insect pest on palm plants in the tropical area of China.In order to find efficient biocontrol agent against O.arenosella,four Metarhizium anisopliae strains with good cultural characters were obtained from dead overwintering O.arenosella larvae collected from Lingshui,Hainan Province.The strains were identified by molecular method and their toxicities against the 3rd instar of O.arenosella were investigated in laboratory.Strain LS-Y1 demonstrated high toxicity against O.arenosella.NCBI BLAST with the rDNA-ITS fragments from the four tested strains revealed that the nucleotide sequences of these genes have the similarity of 99%,100%,99% and99% with the sequences of JN377427.1,NR132017.1,FJ545278.1,FJ545294.1,respectively.LC50 value of LS-Y1 on the 3rd instar larvae of O.arenosella was 4.41×106 conidia/mL6 days after treatment.The LT50 values of LS-Y1 on the 3rd instar larvae of O.arenosella at the spores concentrations of 6.25×106,1.25×107,2.5×107,5×107 and 1×108 conidia/mL were 5.77 d,4.88 d,4.43 d,3.82 d and 3.36 d,respectively.These results showed that the strain LS-Y1 was highly toxic against O.arenosellalarvae,which was the promising candidate in the biological control of O.arenosella.

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