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当前位置: 首页 > 文章 > 产β-葡萄糖苷酶细菌的筛选及转化白藜芦醇的研究 生物技术通报 2017 (11) 130-135
Position: Home > Articles > The Screening of β-Glycosidase-producing Strain and the Transforming of Resveratrol Biotechnology Bulletin 2017 (11) 130-135

产β-葡萄糖苷酶细菌的筛选及转化白藜芦醇的研究

作  者：
冯薇;胡小妍;马明娜;郭萌;路福平;李玉
关键词：
白藜芦醇;沙福芽孢杆菌;β-葡萄糖苷酶;虎杖苷
摘  要：
白藜芦醇具有抗癌、抗氧化等八大功效,在医药、化妆品等领域应用广泛。为获得分泌β-葡萄糖苷酶的细菌菌株,并实现其对虎杖苷的有效转化。通过栀子苷平板初筛、虎杖苷摇瓶复筛,筛选得到一株能够分泌β-葡萄糖苷酶,转化虎杖苷生成白藜芦醇的菌株,并利用16S r DNA序列对筛选得到的菌株进行鉴定,鉴定为沙福芽孢杆菌(Bacillus safensis),命名为CGMCC13129,该菌株在37℃,接种量为7%,底物虎杖苷浓度为0.1%,p H为7,转化8 h的条件下,对底物虎杖苷的转化率可达90%以上,利用HPLC、HPLC-MS、1H-NMR等手段检测转化产物为白藜芦醇,经甲醇萃取一次,纯度高达99.3%。
译  名：
The Screening of β-Glycosidase-producing Strain and the Transforming of Resveratrol
作  者：
FENG Wei;HU Xiao-yan;MA Ming-na;GUO Meng;LU Fu-ping;LI Yu;Key Laboratory of Industrial Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology;
单  位：
Key Laboratory of Industrial Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology
关键词：
resveratrol;;Bacillus safensis;;β-glycosidase enzyme;;polydatin
摘  要：
Resveratrol has a lot of benefits such as anti-cancer and anti-oxidation,and thus is widely applied in cosmetics,medicineand so on. This study aims to screen bacteria which can secrete β-glycosidase and study it's ability of transforming polydatin. Through thegeniposide plate screening and polydatin shake flask,a strain secreting β-glucosidase and transforming polydatin to resveratrol was acquired. Itwas identified as Bacillus safensis by 16 S r DNA sequence analysis,named as CGMCC 13129. Under these conditions of 37℃,with 7 percentof inoculation,p H7,and conversing for 8 h,the conversion rate of polydatin was up to 90%. HPLC,HPLC-MS and 1 H-NMR were used totest and identify the product as resveratrol,and the purity of the product was up to 99.3% by one-time methanol extraction.

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